Lee Yongkyu
Department of Food and Biotechnology, Dongseo University, Busan 617-716, Korea.
Int J Mol Med. 2008 Jun;21(6):765-70.
Epidemiological and experimental carcinogenesis studies provide evidence that certain components of garlic have anti-cancer activity. Although the biotransformed garlic derivative S-allylmercapto-L-cysteine (SAMC) has been reported to show an inhibitory effect on tumorigenesis, the mechanisms are poorly understood. The present study investigated the effect of SAMC on the growth of human gastric cancer SNU-1 cells. Upon treatment with SAMC, a concentration-dependent inhibition of cell proliferation was observed and cells developed many of the hallmark features of apoptosis, including DNA fragmentation and an increase in the sub-diploid population. The anti-proliferative and apoptotic effect of SAMC was associated with the induction of Bax, p53, and caspase-9, rather than the induction of Bcl-2 and p21. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of SAMC, which mediates cell death. These results suggest that the apoptotic effect of SAMC on gastric cancer SNU-1 cells may be connected with caspase-3 activation through the induction of Bax and p53, rather then Bcl-2 and p21.
流行病学和实验性致癌研究表明，大蒜的某些成分具有抗癌活性。尽管据报道生物转化的大蒜衍生物S-烯丙基巯基-L-半胱氨酸（SAMC）对肿瘤发生有抑制作用，但其机制尚不清楚。本研究调查了SAMC对人胃癌SNU-1细胞生长的影响。用SAMC处理后，观察到细胞增殖呈浓度依赖性抑制，并且细胞出现了许多凋亡的标志性特征，包括DNA片段化和亚二倍体群体增加。SAMC的抗增殖和凋亡作用与Bax、p53和caspase-9的诱导有关，而不是与Bcl-2和p21的诱导有关。线粒体细胞色素c激活和体外caspase-3活性测定表明，caspases的激活伴随着SAMC的凋亡作用，后者介导细胞死亡。这些结果表明，SAMC对胃癌SNU-1细胞的凋亡作用可能通过诱导Bax和p53而非Bcl-2和p21与caspase-3激活相关。
