Teufel Katharina, Bleiholder Anne, Griesbach Tim, Pfeifer Felicitas
Institute for Microbiology and Genetics, Technische Universität Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany.
Arch Microbiol. 2008 Sep;190(3):309-18. doi: 10.1007/s00203-008-0383-5. Epub 2008 May 28.
The presence and expression of the multiple tbp genes encoding TATA-box binding proteins (TBPs) was investigated in various strains and mutants of the archaeon Halobacterium salinarum. Six genes, tbpA through tbpF, are present in the genome of Hbt. salinarum NRC-1 and also in the gas vesicle negative mutant strain R1. The only tbp gene located in the chromosome is tbpE, whereas all others are found in the plasmid DNA. Due to the dynamic nature of the plasmids in the Halobacterium strains, the copy numbers of the alternative tbp genes vary significantly. Five tbp genes (tbpA through tbpE) were present in the wild-type strain Hbt. salinarum PHH1. The tbpC gene of Hbt. salinarum PHH1 carried an ISH27-2 insertion element at the start of the reading frame that prevented the expression. All other tbp genes of PHH1 were expressed under aerobic and anaerobic growth conditions and quantitative RT-PCR yielded tbpE as dominant tbp transcript during the exponential growth phase. The plasmid deletion variant Hbt. salinarum PHH4 lacked all of the tbp genes except for tbpE and showed an altered growth behaviour compared to PHH1 wild-type in the stationary growth phase under anaerobic growth conditions.
对嗜盐古菌盐生盐杆菌的各种菌株和突变体中编码TATA框结合蛋白（TBP）的多个tbp基因的存在情况和表达进行了研究。六个基因，即tbpA至tbpF，存在于盐生盐杆菌NRC-1的基因组中，也存在于气荚膜阴性突变体菌株R1中。位于染色体上的唯一tbp基因是tbpE，而其他所有基因都存在于质粒DNA中。由于盐杆菌菌株中质粒的动态性质，替代tbp基因的拷贝数差异很大。野生型菌株盐生盐杆菌PHH1中存在五个tbp基因（tbpA至tbpE）。盐生盐杆菌PHH1的tbpC基因在阅读框起始处携带一个ISH27-2插入元件，阻止了其表达。PHH1的所有其他tbp基因在有氧和无氧生长条件下均有表达，定量RT-PCR结果显示在指数生长期tbpE是主要的tbp转录本。质粒缺失变体盐生盐杆菌PHH4除tbpE外缺少所有tbp基因，并且在厌氧生长条件下的稳定生长期与PHH1野生型相比表现出改变的生长行为。
