Pfeiffer F, Schuster S C, Broicher A, Falb M, Palm P, Rodewald K, Ruepp A, Soppa J, Tittor J, Oesterhelt D
Department of Membrane Biochemistry, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Genomics. 2008 Apr;91(4):335-46. doi: 10.1016/j.ygeno.2008.01.001. Epub 2008 Mar 3.
We report the sequence of the Halobacterium salinarum strain R1 chromosome and its four megaplasmids. Our set of protein-coding genes is supported by extensive proteomic and sequence homology data. The structures of the plasmids, which show three large-scale duplications (adding up to 100 kb), were unequivocally confirmed by cosmid analysis. The chromosome of strain R1 is completely colinear and virtually identical to that of strain NRC-1. Correlation of the plasmid sequences revealed 210 kb of sequence that occurs only in strain R1. The remaining 350 kb shows virtual sequence identity in the two strains. Nevertheless, the number and overall structure of the plasmids are largely incompatible. Also, 20% of the protein sequences differ despite the near identity at the DNA sequence level. Finally, we report genome-wide mobility data for insertion sequences from which we conclude that strains R1 and NRC-1 originate from the same natural isolate. This exemplifies evolution in the laboratory.
我们报道了盐沼盐杆菌R1菌株染色体及其四个大质粒的序列。我们的蛋白质编码基因集得到了广泛的蛋白质组学和序列同源性数据的支持。通过粘粒分析明确证实了质粒的结构，这些质粒显示出三个大规模重复（总计100 kb）。R1菌株的染色体完全共线，实际上与NRC-1菌株的染色体相同。质粒序列的相关性揭示了仅在R1菌株中出现的210 kb序列。其余350 kb在两个菌株中显示出几乎相同的序列。然而，质粒的数量和总体结构在很大程度上不兼容。此外，尽管在DNA序列水平上几乎相同，但仍有20%的蛋白质序列不同。最后，我们报告了插入序列的全基因组迁移数据，由此得出R1和NRC-1菌株源自同一自然分离株的结论。这例证了实验室中的进化。
