Cong Long-Bo, Yuan Hai-Long, Wang Qi, Li Xian-Yi, Gong Qian-Feng, Xiao Xiao-He
302 Hospital of PLA, Institute of PLA of Chinese Medicine, Beijing 100039, People's Republic of China.
Biomed Chromatogr. 2008 Oct;22(10):1084-90. doi: 10.1002/bmc.1028.
A reversed-phase high-performance liquid chromatographic method with diode array detection was established to simultaneously determine the seven bioactive lignans in Herpetospermum caudigerum, namely ent-isolariciresinol (1), dehydrodiconiferyl alcohol (2), herpetrione (3), herpetin (4), herpetetrone (5), herpetotriol (6) amd herpetal (7). The HPLC assay was performed on a Restek Pinnacle DB C18 column (250 x 4.6 mm, 5 microm) with gradient elution of acetonitrile and 0.1% phosphoric acid within 65 min. The detection wavelength was 240 nm. The flow-rate was 1.0 mL/min. All calibration curves showed good linearity (r2 > 0.9998) within test ranges. The method was reproducible with intra- and inter-day variation of less than 1.98%. The method provided good accuracy with recoveries in the range 95.19-102.64% with RSDs less than 1.52%. The method was successfully applied to the quantification of seven constituents in 15 H. caudigerum samples collected from different cities. The results indicated that the developed assay could be considered as a suitable quality control method for H. caudigerum.
建立了一种具有二极管阵列检测的反相高效液相色谱法，用于同时测定藏药波棱瓜子中的七种生物活性木脂素，即对映异落叶松脂醇（1）、脱氢二松柏醇（2）、波棱酮（3）、波棱素（4）、波棱特酮（5）、波棱三醇（6）和波棱花瓣素（7）。高效液相色谱分析在Restek Pinnacle DB C18柱（250×4.6 mm，5μm）上进行，乙腈和0.1%磷酸在65分钟内梯度洗脱。检测波长为240 nm。流速为1.0 mL/min。所有校准曲线在测试范围内均显示出良好的线性（r2>0.9998）。该方法具有可重复性，日内和日间变化小于1.98%。该方法具有良好的准确性，回收率在95.19-102.64%范围内，相对标准偏差小于1.52%。该方法成功应用于从不同城市采集的15份波棱瓜子样品中七种成分的定量分析。结果表明，所建立的分析方法可被视为波棱瓜子合适的质量控制方法。
