高效液相色谱法定量测定灵芝及相关物种中的六种主要三萜类化合物

Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography.

作者信息

Wang Xiao-Ming, Yang Min, Guan Shu-Hong, Liu Rong-Xia, Xia Jia-Meng, Bi Kai-Shun, Guo De-An

机构信息

Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institute of Biological Sciences, Guo Shoujing Road 199, Zhangjiang, Shanghai 201203, PR China.

出版信息

J Pharm Biomed Anal. 2006 Jun 7;41(3):838-44. doi: 10.1016/j.jpba.2006.01.053. Epub 2006 Mar 10.

DOI:10.1016/j.jpba.2006.01.053

PMID:16530372

Abstract

A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mmx4.6 mm, 5 microm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2>0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.

摘要

建立了一种反相液相色谱法，用于定量测定灵芝及其近缘种中的六种三萜类化合物，即灵芝酸C2、B、AM1、K、H和D。样品在超声浴中用氯仿萃取。在Agilent Zorbax SB-C18柱（250 mm×4.6 mm，5μm）上实现了分离和检测的最佳条件，以乙腈和0.03%磷酸水溶液（v/v）进行线性梯度洗脱，流速为1.0 ml/min，检测波长为252 nm。所有校准曲线在测试范围内均显示出良好的线性（r2>0.999）。该方法日内和日间测定的相对偏差小于2%，方法回收率为93-103%，相对标准偏差（R.S.D.）小于5%。将该测定方法应用于36个不同灵芝及其近缘种样品中三萜类成分的定量测定。结果表明，所建立的方法可作为灵芝及其近缘种的质量控制方法。