黄酮类化合物增强Ru(bpy)3(2+)的电化学发光

Enhanced electrochemiluminescence of Ru(bpy)3(2+) by flavone compounds.

作者信息

Lin Zhenyu, Chen Jinhua, Chen Guonan

机构信息

Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, People's Republic of China.

出版信息

Luminescence. 2008 Nov-Dec;23(6):365-9. doi: 10.1002/bio.1045.

DOI:10.1002/bio.1045

PMID:18506909

Abstract

Flavones such as morin, rutin, quercitrin, quercetin and wogonin were found to be able to strongly enhance the electrochemiluminescence (ECL) of the Ru(bpy)(3)(2+) system. Based on this, a novel ECL method with good stability and reproducibility could be developed for determination of flavones. Under the optimum conditions, the enhanced ECL intensity was linear with the flavones concentration in a wide range. The detection limits (defined as S:N = 3) for morin, rutin, quercitrin, quercetin and wogonin were 3.2 x 10(-7) mol/L, 4.3 x 10(-7), 1.8 x 10(-7), 8.0 x 10(-8) and 1.0 x 10(-7 ) mol/L, respectively. In addition, the possible mechanism for the Ru(bpy)(3)(2+) ECL system in the presence of flavones is also discussed.

摘要

发现诸如桑色素、芦丁、槲皮苷、槲皮素和汉黄芩素等黄酮类化合物能够显著增强Ru(bpy)(3)(2+)体系的电化学发光（ECL）。基于此，可开发出一种稳定性和重现性良好的新型ECL方法用于测定黄酮类化合物。在最佳条件下，增强的ECL强度在很宽的范围内与黄酮类化合物浓度呈线性关系。桑色素、芦丁、槲皮苷、槲皮素和汉黄芩素的检测限（定义为S:N = 3）分别为3.2 x 10(-7) mol/L、4.3 x 10(-7)、1.8 x 10(-7)、8.0 x 10(-8)和1.0 x 10(-7 ) mol/L。此外，还讨论了存在黄酮类化合物时Ru(bpy)(3)(2+) ECL体系的可能机理。