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转化生长因子-β1诱导ML-CCl64细胞中cAMP反应元件结合蛋白的磷酸化。

TGF-beta 1 induces phosphorylation of the cyclic AMP responsive element binding protein in ML-CCl64 cells.

作者信息

Kramer I M, Koornneef I, de Laat S W, van den Eijnden-van Raaij A J

机构信息

Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands.

出版信息

EMBO J. 1991 May;10(5):1083-9. doi: 10.1002/j.1460-2075.1991.tb08048.x.

DOI:10.1002/j.1460-2075.1991.tb08048.x
PMID:1850693
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC452761/
Abstract

Type beta transforming growth factors represent a family of polypeptides that modulate growth and differentiation. TGF-beta exerts its effects on target cells through interaction with specific cell surface receptors, but the signal transduction pathways are largely unresolved as yet. In this study we report that TGF-beta 1 induces a rapid phosphorylation of the cyclic AMP responsive element binding protein (CREB) in mink lung CCl64 cells. Phosphorylation induced by TGF-beta 1 is not mediated by the cAMP-dependent protein kinase. Parallel to the increase in phosphorylation of CREB, an increase in binding to the collagenase TPA responsive element was observed. CREB participates in the binding to this element, probably as a heterodimer with another as yet unknown protein. The modification imposed on CREB and its involvement in an enhanced TRE-binding could be a mechanism by which TGF-beta 1 induces the TRE-mediated transcriptional activation.

摘要

β型转化生长因子是一类调节生长和分化的多肽家族。转化生长因子-β（TGF-β）通过与特定细胞表面受体相互作用对靶细胞发挥作用，但其信号转导途径目前很大程度上仍未明确。在本研究中，我们报告TGF-β1可诱导水貂肺CCl64细胞中环磷酸腺苷反应元件结合蛋白（CREB）的快速磷酸化。TGF-β1诱导的磷酸化不是由环磷酸腺苷依赖性蛋白激酶介导的。与CREB磷酸化增加平行，观察到与胶原酶佛波酯反应元件（TPA responsive element, TRE）的结合增加。CREB可能作为与另一种未知蛋白质的异二聚体参与与该元件的结合。对CREB的修饰及其参与增强的TRE结合可能是TGF-β1诱导TRE介导的转录激活的一种机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/6ad9de02315c/emboj00103-0062-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/25c981c5d836/emboj00103-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/5f797c3eeb1b/emboj00103-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/b900bf4c7ac1/emboj00103-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/56a377b59873/emboj00103-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/f55dfa8d833d/emboj00103-0061-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/38da17d2a52a/emboj00103-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa7b/452761/6ad9de02315c/emboj00103-0062-b.jpg

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本文引用的文献

1
FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA.FBJ小鼠骨肉瘤病毒：具有生物活性的前病毒DNA的鉴定与分子克隆
J Virol. 1982 Nov;44(2):674-82. doi: 10.1128/JVI.44.2.674-682.1982.
2
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳研究乳糖阻遏物-操纵基因相互作用的平衡与动力学
Nucleic Acids Res. 1981 Dec 11;9(23):6505-25. doi: 10.1093/nar/9.23.6505.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
蛋白激酶 A 抑制剂 H89 可减轻实验性增生性玻璃体视网膜病变。
Invest Ophthalmol Vis Sci. 2020 Feb 7;61(2):1. doi: 10.1167/iovs.61.2.1.
4
The Discovery and Early Days of TGF-β: A Historical Perspective.转化生长因子-β的发现与早期岁月：历史视角
Cold Spring Harb Perspect Biol. 2016 Jul 1;8(7):a021865. doi: 10.1101/cshperspect.a021865.
5
Transforming growth factor-beta1 causes pulmonary microvascular endothelial cell apoptosis via ALK5.转化生长因子-β1 通过激活素受体样激酶 5 导致肺微血管内皮细胞凋亡。
Am J Physiol Lung Cell Mol Physiol. 2009 May;296(5):L825-38. doi: 10.1152/ajplung.90307.2008. Epub 2009 Mar 6.
6
Cross-talk between signaling pathways in murine embryonic palate cells: effect of TGF beta and cAMP on EGF-induced DNA synthesis.小鼠胚胎腭细胞中信号通路间的相互作用：转化生长因子β和环磷酸腺苷对表皮生长因子诱导的DNA合成的影响
In Vitro Cell Dev Biol Anim. 1998 Jan;34(1):74-8. doi: 10.1007/s11626-998-0056-3.
7
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10
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4
Viral and cellular fos proteins are complexed with a 39,000-dalton cellular protein.病毒和细胞的原癌基因Fos蛋白与一种39000道尔顿的细胞蛋白复合。
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5
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6
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7
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8
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9
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