Schrold J, Nedergaard O A
Eur J Pharmacol. 1976 Oct;39(2):423-7. doi: 10.1016/0014-2999(76)90156-4.
Electrical field stimulation of the isolated adventitial and intima-medial layers of rabbit aorta preloaded with 3H-noradrenaline elicited a 3H outflow. The initial 3H outflow from adventitia was independent of external Ca2+ concentration. Each of the subsequent 3H outflows was mainly Ca2+ sensitive. All stimulation-induced 3H outflows from media were Ca2+ insensitive. It is concluded that the isolated adventitia is a suitable preparation to outflow from aorta is of neuronal and extraneuronal origin.
对预先加载了3H-去甲肾上腺素的兔主动脉分离的外膜和内膜-中层进行电场刺激，引发了3H流出。外膜最初的3H流出与细胞外Ca2+浓度无关。随后的每次3H流出主要对Ca2+敏感。来自中层的所有刺激诱导的3H流出对Ca2+不敏感。结论是，分离的外膜是一种合适的制备物，可用于研究源自神经元和非神经元的主动脉流出。
