Wimmer Eva, Kraehn-Senftleben Gertraud, Issing Wolfgang J
Department of Otorhinolaryngology, Head and Neck Surgery, Ludwig-Maximilians-University, Munich, Germany.
Anticancer Res. 2008 Mar-Apr;28(2A):973-9.
In order to elucidate the role of the receptor tyrosine kinase HER3, the expression characteristics in different tissues of cutaneous malignancies and in normal skin were compared.
In this study HER3 expression was evaluated by RT-PCR analysis and immunohistochemistry from different tissue specimens of cutaneous tumors like nevi, primary malignant melanomas, basal cell carcinoma, squamous cell carcinoma and malignant melanoma metastases and normal skin samples and graded into weak, moderate and strong expression. Associations of tumor thickness in these specimens with HER3 expressions were also analyzed.
HER3 expression was found in 63% (10/16) of the basal cell carcinomas, in 4/5 of squamous cell carcinomas and in one Merkel cell carcinoma. Within the group of different malignant melanomas, HER3 expression was detected in 35% of the nodular malignant melanomas (6/17) and in 9/19 of the superficial spreading melanomas, including 2 lentigo malignant melanomas. The majority of melanomas with a higher tumor thickness expressed HER3, and 85% of melanoma metastasis were HER3-positive.
HER3 expression was associated with hyperproliferate tumor stages and suggested that HER3 expression could reflect an increased malignant potential in cutaneous lesions.
为阐明受体酪氨酸激酶HER3的作用，比较了其在皮肤恶性肿瘤不同组织及正常皮肤中的表达特征。
本研究通过逆转录聚合酶链反应（RT-PCR）分析和免疫组织化学方法，对痣、原发性恶性黑色素瘤、基底细胞癌、鳞状细胞癌、恶性黑色素瘤转移灶等皮肤肿瘤不同组织标本以及正常皮肤样本中的HER3表达进行评估，并分为弱、中、强表达。同时分析了这些标本中肿瘤厚度与HER3表达的相关性。
在63%（10/16）的基底细胞癌、4/5的鳞状细胞癌和1例默克尔细胞癌中发现HER3表达。在不同类型的恶性黑色素瘤中，35%的结节性恶性黑色素瘤（6/17）和9/19的浅表扩散性黑色素瘤（包括2例雀斑样恶性黑色素瘤）检测到HER3表达。大多数肿瘤厚度较高的黑色素瘤表达HER3，85%的黑色素瘤转移灶HER3呈阳性。
HER3表达与肿瘤增殖活跃阶段相关，提示HER3表达可能反映皮肤病变中恶性潜能的增加。
