Lu Kung-Wen, Tsai Ming-Li, Chen Jung-Chou, Hsu Shu-Chun, Hsia Te-Chun, Lin Meng-Wei, Huang An-Cheng, Chang Yung-Hsien, Ip Siu-Wan, Lu Hsu-Feng, Chung Jing-Gung
Chinese Medical Research Institute, China Medical University, Taichung, Taiwan, ROC.
Anticancer Res. 2008 Mar-Apr;28(2A):1093-9.
Gypenosides (Gyp), components of Gynostemma pentaphyllum Makino, were found to induce suppression of human tongue squamous cell carcinoma SCC4 cell growth and induce apoptosis in response to overexpression of reactive oxygen species, calcium (Ca(+2)) and to decrease mitochondrial membrane potential in vitro. In this study, the effect of Gyp on cell migration and invasion of human tongue SCC4 cells was examined. SCC4 cells treated in vitro with Gyp migrated and invaded less than cells treated with phosphate-buffered saline (PBS) as a control. Gyp inhibited migration and invasion by down-regulating the production of RAS, NFkappaB, COX2, ERK1/2 and MMP-9 relative to PBS only. These results show that Gyp inhibits invasion and migration of human tongue SCC4 cells by down-regulating proteins associated with these processes, resulting in reduced metastasis.
绞股蓝皂苷（Gyp）是绞股蓝的成分，已发现其可抑制人舌鳞状细胞癌SCC4细胞的生长，并在体外因活性氧、钙（Ca(+2)）的过表达而诱导细胞凋亡，还可降低线粒体膜电位。在本研究中，检测了Gyp对人舌SCC4细胞迁移和侵袭的影响。体外经Gyp处理的SCC4细胞，其迁移和侵袭能力低于作为对照的经磷酸盐缓冲盐水（PBS）处理的细胞。相对于仅用PBS处理的细胞，Gyp通过下调RAS、NFκB、COX2、ERK1/2和MMP-9的产生来抑制迁移和侵袭。这些结果表明，Gyp通过下调与这些过程相关的蛋白质来抑制人舌SCC4细胞的侵袭和迁移，从而减少转移。
