Pei Hui-Lin, Mu Dan-Mei, Zhang Bin
Department of Obstetrics, General Hospital of Daqing Oilfield, Daqing, Heilongjiang, China (mainland).
Department of Science and Education, General Hospital of Daqing Oilfield, Daqing, Heilongjiang, China (mainland).
Med Sci Monit. 2017 Jun 30;23:3192-3199. doi: 10.12659/msm.901833.
BACKGROUND Epithelial ovarian cancer is a major cause of mortality in women and one of the most common gynecologic disorders. Pterostilbene (PTS), a trans-3,5-dimethoxy-4'-hydroxystilbene, was chosen for this work due to its reported effectiveness as a chemotherapeutic agent in cancer studies. In this work, we studied underlying molecular mechanisms of PTS treatment in various ovarian cancer cell lines such as OVCAR8, OV1063, IGROV-1, and SKOV3. MATERIAL AND METHODS We used the cytometric bead array (CBA) method and real-time PCR analysis to analyze the secretion level of tumor necrosis factor alpha (TNF-α) and to measure the TNF-α mRNA expression. NF-kappa B (NF-κB) promoter analysis, Western blot analysis, electrophoresis mobility shift assay (EMSA), and immunostaining analyses were performed to measure the NF-κB activity and other relative proteins levels. RESULTS The PTS treatment decreased the release of TNF-α in IGROV-1 ovarian cancer cells. It also showed significant inhibitory effect on nuclear NF-κB p50, and NF-κB p65 protein levels. CONCLUSIONS From the results obtained, we suggest that PTS has the potential to treat ovarian cancer by reducing the level of TNF-α cytokine and to have a limited effect on NF-κB, AKT, and ERK signaling pathways.
上皮性卵巢癌是女性死亡的主要原因之一，也是最常见的妇科疾病之一。紫檀芪（PTS），一种反式-3,5-二甲氧基-4'-羟基芪，因其在癌症研究中作为化疗药物的有效性而被选用于本研究。在这项研究中，我们研究了PTS处理在各种卵巢癌细胞系（如OVCAR8、OV1063、IGROV-1和SKOV3）中的潜在分子机制。
我们使用细胞计数珠阵列（CBA）方法和实时PCR分析来分析肿瘤坏死因子α（TNF-α）的分泌水平，并测量TNF-α mRNA表达。进行NF-κB启动子分析、蛋白质印迹分析、电泳迁移率变动分析（EMSA）和免疫染色分析以测量NF-κB活性和其他相关蛋白水平。
PTS处理降低了IGROV-1卵巢癌细胞中TNF-α的释放。它还对核NF-κB p50和NF-κB p65蛋白水平显示出显著的抑制作用。
从获得的结果来看，我们认为PTS有可能通过降低TNF-α细胞因子水平来治疗卵巢癌，并且对NF-κB、AKT和ERK信号通路有有限的影响。
