兔骨髓间充质干细胞接种生物衍生骨材料联合重组人骨形态发生蛋白-2治疗股骨头坏死

Treatment of osteonecrosis of femoral head with BMSCs-seeded bio-derived bone materials combined with rhBMP-2 in rabbits.

作者信息

Xiao Zeng-ming, Jiang Hua, Zhan Xin-li, Wu Zhen-guo, Zhang Xing-lin

机构信息

Department of Orthopedics, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.

出版信息

Chin J Traumatol. 2008 Jun;11(3):165-70. doi: 10.1016/s1008-1275(08)60035-8.

DOI:10.1016/s1008-1275(08)60035-8

PMID:18507947

Abstract

OBJECTIVE

To evaluate the effect of autologous bone marrow mesenchymal stem cells (BMSCs) seeded bio-derived bone materials (BBM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in repairing defect of osteonecrosis of femoral head (ONFH).

METHODS

Early-stage osteonecrosis in the left hip was induced in 36 adult New Zealand white rabbits (provided by the Animal Center of Guangxi Medical University, Nanning, China) after core decompression and delivery of liquid nitrogen into the femoral head. Then the animals were divided into three groups according to the type of implants for bone repair: 12 rabbits with nothing (Group I, the blank control group), 12 with BBM combined with rhBMP-2 (Group II), and 12 with BMSCs-seeded BBM combined with rhBMP-2 (Group III). At 4, 8, and 12 weeks after surgery, X-ray of the femoral head of every 4 rabbits in each group was taken, and then they were killed and the femoral heads were collected at each time point, respectively. Gross observation was made on the femoral heads. After hematoxylin and eosin staining, Lane-sandhu scores of X-ray and bone densitometry were calculated and the histomorphometric measurements were made for the new bone trabeculae.

RESULTS

At 12 weeks after surgery, two femoral heads collapsed in Group I, but none in Group II or Group III. X-ray examination showed that the femoral heads in Group I had defect shadow or collapsed while those in Group II had a low density and those in Group III presented with a normal density. Histologically, the defects of femoral heads were primarily filled with no new bone but fibrous tissues in Group I. In contrast, new bone regeneration and fibrous tissues occurred in Group II and only new bone regeneration occurrd in Group III. Lane-sandhu scores of X-ray, bone mineral density and rate of new bone in trabecular area in Group III were higher significantly than those of the other two groups.

CONCLUSIONS

Our findings indicate a superior choice of repairing the experimental defect of ONFH with BMSCs- seeded BBM combined with rhBMP-2.

摘要

目的

评估自体骨髓间充质干细胞（BMSCs）接种于生物衍生骨材料（BBM）并联合重组人骨形态发生蛋白-2（rhBMP-2）修复股骨头坏死（ONFH）缺损的效果。

方法

对36只成年新西兰白兔（由中国南宁广西医科大学动物中心提供）进行股骨头髓芯减压并注入液氮，诱导左髋早期坏死。然后根据骨修复植入物类型将动物分为三组：12只不做处理（I组，空白对照组），12只植入BBM联合rhBMP-2（II组），12只植入BMSCs接种的BBM联合rhBMP-2（III组）。术后4、8、12周，每组每4只兔子拍摄股骨头X线片，然后处死并分别在各时间点收集股骨头。对股骨头进行大体观察。苏木精-伊红染色后，计算X线的Lane-sandhu评分和骨密度，并对新骨小梁进行组织形态计量学测量。

结果

术后12周，I组有2个股骨头塌陷，II组和III组无塌陷。X线检查显示，I组股骨头有缺损阴影或塌陷，II组股骨头密度低，III组股骨头密度正常。组织学上，I组股骨头缺损主要填充无新骨的纤维组织。相比之下，II组出现新骨再生和纤维组织，III组仅出现新骨再生。III组X线的Lane-sandhu评分、骨矿物质密度和小梁区新骨率显著高于其他两组。