Vincensini Laetitia, Fall Gamou, Berry Laurence, Blisnick Thierry, Braun Breton Catherine
Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 2581 Institut Pasteur, 25-28 Rue du Dr Roux, Paris, France.
Mol Biochem Parasitol. 2008 Aug;160(2):81-9. doi: 10.1016/j.molbiopara.2008.04.002. Epub 2008 Apr 12.
The high-molecular mass rhoptry protein complex (PfRhopH), which comprises three distinct gene products, RhopH1, RhopH2, and RhopH3, is known to be secreted and transferred to the parasitophorous vacuole membrane upon invasion of a red blood cell by the malaria parasite Plasmodium falciparum. Here we show that the merozoite-acquired RhopH complex is also transferred to defined domains of the red blood cell cytoplasm, and possibly transiently associated with Maurer's clefts. This is the first report of trafficking in the host cell cytoplasm for P. falciparum rhoptry proteins secreted upon red blood cell invasion. Based on its newly identified sub-cellular location and the phenotype of RhopH1 mutants, we propose that the RhopH complex participate in the assembly of the cytoadherence complex.
高分子量棒状体蛋白复合物（PfRhopH）由三种不同的基因产物RhopH1、RhopH2和RhopH3组成，已知在恶性疟原虫侵入红细胞后会被分泌并转移至寄生泡膜。在此我们表明，裂殖子获得的RhopH复合物也会转移至红细胞细胞质的特定区域，并可能短暂地与毛氏小体相关联。这是关于恶性疟原虫侵入红细胞后分泌的棒状体蛋白在宿主细胞质中运输的首次报道。基于其新确定的亚细胞定位以及RhopH1突变体的表型，我们提出RhopH复合物参与细胞黏附复合物的组装。
