使用同步调制作为电损伤细胞的挽救模型，从电穿孔中实现细胞恢复。

Cellular recovery from electroporation using synchronisation modulation as a rescue model for electrically injured cells.

作者信息

Dando Robin, Chen Wei

机构信息

Laboratory for Cellular and Molecular Biophysics, Department of Physics, University of South Florida, Tampa, FL 33620, USA.

出版信息

Burns. 2008 Dec;34(8):1128-36. doi: 10.1016/j.burns.2008.01.020. Epub 2008 May 27.

DOI:10.1016/j.burns.2008.01.020

PMID:18508201

Abstract

Electroporation of the plasma membrane resulting in a decrement in transmembrane potential is offered as a model in the study of the rescuing effects of the synchronisation modulation technique by electrically activating sodium potassium adenosine triphosphatase. Living cells were first electrically damaged by a pulsed intensive electric field, resulting in cell membrane electroporation, ion leakages and membrane potential depolarisation. Their recovery rate in natural conditions was compared with that of cells in a synchronisation modulation electric field. Fluorescence readings were taken using confocal microscopy and a potentiometric dye. Significantly more rapid recovery was observed after synchronisation modulation, with cell membranes actually polarised to levels higher than the original resting potential, a feature never seen in naturally recovering cells.

摘要

质膜电穿孔导致跨膜电位降低，被作为一种模型用于研究通过电激活钠钾三磷酸腺苷酶的同步调制技术的拯救效果。活细胞首先受到脉冲强电场的电损伤，导致细胞膜电穿孔、离子泄漏和膜电位去极化。将它们在自然条件下的恢复率与同步调制电场中的细胞恢复率进行比较。使用共聚焦显微镜和电位染料进行荧光读数。同步调制后观察到恢复明显更快，细胞膜实际上极化到高于原始静息电位的水平，这是自然恢复细胞中从未见过的特征。