Ramos Airton, Suzuki Daniela Ota Hisayasu, Marques Jefferson Luiz Brum
Institute of Biomedical Engineering, Department of Electrical Engineering, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, Brazil.
Artif Organs. 2004 Apr;28(4):357-61. doi: 10.1111/j.1525-1594.2004.47355.x.
This article presents a numerical study of the electroporation process of spherical cells suspended in an electrolyte solution, using the equivalent circuit method (ECM) for field calculation proposed by Ramos et al. A model for the electric conductance of the cell membrane derived from the analytical and experimental results obtained by Glaser et al. for planar lipidic membranes was applied. The influence of the cell concentration and membrane properties (described in the model) on the membrane current, membrane potential, and dependence of the electrolyte conductivity on the applied electric field was studied. These results clarify the electric events connected with the pore opening process and allow the planning of experimental approaches to study reversible membrane rupture in real cells.
本文采用拉莫斯等人提出的用于场计算的等效电路方法（ECM），对悬浮在电解质溶液中的球形细胞的电穿孔过程进行了数值研究。应用了一个基于格拉泽等人对平面脂质膜所获得的分析和实验结果推导出来的细胞膜电导模型。研究了细胞浓度和膜特性（在模型中描述）对膜电流、膜电位以及电解质电导率与外加电场的依赖性的影响。这些结果阐明了与孔开放过程相关的电事件，并有助于规划实验方法来研究真实细胞中的可逆膜破裂。
