La Gatta Annalisa, Schiraldi Chiara, Esposito Annaclaudia, D'Agostino Antonella, De Rosa Alfredo
Department of Experimental Medicine, Faculty of Medicine, Second University of Naples, Via L. De Crecchio 7, 80138 Naples, Italy.
J Biomed Mater Res A. 2009 Jul;90(1):292-302. doi: 10.1002/jbm.a.32094.
Novel p(HEMA-co-METAC)/alginate semi-interpenetrating hydrogels (semi-IPNs) were developed in the attempt to improve poly(hydroxyethylmethacrylate) biological response, extending its applications in the biomedical field. Materials with different alginate contents were synthesized by copolymerization of 2-hydroxyethylmethacrylate and 2-methacryloxy ethyltrimethyl ammonium chloride monomers in the presence of aqueous solutions of alginate and characterized with respect to p(HEMA-co-METAC) synthesized in the presence of water. Swelling studies in water revealed high values of water uptake (>100%) with marked differences in the swelling degree at increasing polysaccharide content. The effect of ionic strength and of pH on the swelling behavior of hydrogels was also investigated. Higher ionic strengths resulted in a minor swelling degree accordingly with hydrogels polyelectrolyte nature. The introduction of the natural ionizable polysaccharide into the network made the semi-IPNs swelling depending on pH values of the solutions. A biological characterization was performed in terms of protein absorption on hydrogel surfaces, cytotoxicity (ISO 10993-5) and cell adhesion and proliferation studies using both murine 3T3 and human fibroblasts. Hydrogels proved noncytotoxic; moreover, semi-IPN surfaces allowed cell attachment and proliferation, thus supporting their potential biomedical use.
新型聚（甲基丙烯酸羟乙酯 - 共 - 甲基丙烯酰氧乙基三甲基氯化铵）/海藻酸盐半互穿水凝胶（半互穿聚合物网络）被开发出来，旨在改善聚甲基丙烯酸羟乙酯的生物学反应，拓展其在生物医学领域的应用。通过在海藻酸盐水溶液存在下使甲基丙烯酸羟乙酯和甲基丙烯酰氧乙基三甲基氯化铵单体共聚，合成了具有不同海藻酸盐含量的材料，并对其进行了表征，同时也对在水存在下合成的聚（甲基丙烯酸羟乙酯 - 共 - 甲基丙烯酰氧乙基三甲基氯化铵）进行了表征。在水中的溶胀研究表明，吸水率很高（>100%），并且随着多糖含量的增加，溶胀程度存在显著差异。还研究了离子强度和pH值对水凝胶溶胀行为的影响。较高的离子强度导致溶胀程度较小，这与水凝胶的聚电解质性质相符。将天然可电离多糖引入网络后，半互穿聚合物网络的溶胀取决于溶液的pH值。通过水凝胶表面的蛋白质吸附、细胞毒性（ISO 10993 - 5）以及使用小鼠3T3细胞和人成纤维细胞进行的细胞黏附与增殖研究进行了生物学表征。水凝胶被证明无细胞毒性；此外，半互穿聚合物网络表面允许细胞附着和增殖，因此支持它们在生物医学方面的潜在用途。
