Ruiz-Herrera José, Ortiz-Castellanos Lucila, Martínez Ana I, León-Ramírez Claudia, Sentandreu Rafael
Departamento de Ingeniería Genética, Unidad Irapuato, CINVESTAV del IPN, Apartado Postal 629, 36500 Irapuato, Gto., Mexico.
Fungal Genet Biol. 2008 Aug;45 Suppl 1:S71-6. doi: 10.1016/j.fgb.2008.04.010. Epub 2008 Apr 24.
A study of the proteins involved in the synthesis and structure of the cell wall of Ustilago maydis was made by in silico analysis of the fungal genome, with reference to supporting experimental evidence. The composition of the cell wall of U. maydis shows similarities with the structural composition of the walls of Ascomycetes, but also shows important differential features. Accordingly, the enzymes involved in the synthesis of the U. maydis wall polysaccharides chitin and beta-1,6 glucans displayed some differential characteristics. The most salient difference in protein composition was the predicted absence of Pir proteins, an important class of proteins present in the Ascomycetes. Other classes of proteins that are covalently-linked to the wall in Ascomycetes, including those bound through disulfide linkages, joined by alkali-labile bonds, and GPI proteins, were predicted to be present in the U. maydis walls. The main characteristic of the exo-cellular, non-covalently-bound proteins was their relative low number, especially for hydrolytic enzymes.
通过对真菌基因组进行电子分析，并参考支持性实验证据，对参与玉米黑粉菌细胞壁合成和结构的蛋白质进行了研究。玉米黑粉菌细胞壁的组成与子囊菌细胞壁的结构组成有相似之处，但也显示出重要的差异特征。因此，参与玉米黑粉菌壁多糖几丁质和β-1,6-葡聚糖合成的酶表现出一些差异特性。蛋白质组成最显著的差异是预测不存在Pir蛋白，这是子囊菌中一类重要的蛋白质。其他在子囊菌中与壁共价连接的蛋白质类别，包括那些通过二硫键结合、通过碱不稳定键连接的蛋白质以及GPI蛋白，预计存在于玉米黑粉菌的细胞壁中。细胞外非共价结合蛋白质的主要特征是其数量相对较少，尤其是水解酶。
