原发性干燥综合征中唾液腺毒蕈碱型乙酰胆碱受体自身抗体结合的证明。

Demonstration of autoantibody binding to muscarinic acetylcholine receptors in the salivary gland in primary Sjögren's syndrome.

作者信息

Kovács László, Fehér Erzsébet, Bodnár Ibolya, Marczinovits Ilona, Nagy György M, Somos János, Boda Viktória

机构信息

1st Department of Internal Medicine, Saint George Hospital of Fejér County, Székesfehérvár, Hungary.

出版信息

Clin Immunol. 2008 Aug;128(2):269-76. doi: 10.1016/j.clim.2008.04.001. Epub 2008 May 27.

DOI:10.1016/j.clim.2008.04.001

Abstract

A significant pathogenetic role of antimuscarinic acetylcholine receptor-3 (anti-m3AChR) autoantibodies in primary Sjögren's syndrome (pSS) has been suggested. However, the binding of these antibodies to the receptors in the target tissues has not yet been demonstrated. In this study, the binding characteristics of pSS sera and anti-m3AChR-monospecific sera affinity-purified from pSS patients to labial salivary gland samples from healthy subjects were studied with light- and electron microscopy. Furthermore, the ultrastructural localisation of in vivo deposited antibodies in pSS salivary glands was also investigated. Light microscopic immunohistochemistry revealed the binding of the anti-m3AChR-specific sera to the membrane of acinar cells. Similar reaction end-products were observed in the pSS salivary gland epithelial cell membranes. With electron microscopy, the autoantibody binding was observed to be localised to the junctions of epithelial cell membranes with nerve endings, both in normal and pSS glands. The results indicate that anti-m3AChR antibodies bind to the receptors in the salivary glands.

摘要

抗毒蕈碱型乙酰胆碱受体3（anti - m3AChR）自身抗体在原发性干燥综合征（pSS）中具有重要的致病作用，这一点已得到提示。然而，这些抗体与靶组织中受体的结合尚未得到证实。在本研究中，利用光学显微镜和电子显微镜研究了pSS患者血清以及从pSS患者中亲和纯化得到的抗m3AChR单特异性血清与健康受试者唇腺样本的结合特性。此外，还研究了pSS唾液腺中体内沉积抗体的超微结构定位。光学显微镜免疫组织化学显示抗m3AChR特异性血清与腺泡细胞膜结合。在pSS唾液腺上皮细胞膜中也观察到类似的反应终产物。通过电子显微镜观察到，在正常和pSS腺体中，自身抗体结合均定位于上皮细胞膜与神经末梢的连接处。结果表明抗m3AChR抗体与唾液腺中的受体结合。