磷酸二酯酶5通过环磷酸鸟苷-环磷酸腺苷而非环磷酸鸟苷-蛋白激酶G信号通路调节卵母细胞自发成熟。
PDE5 modulates oocyte spontaneous maturation via cGMP-cAMP but not cGMP-PKG signaling.
作者信息
Wang Songbo, Ning Gang, Chen Xiufen, Yang Jiange, Ouyang Hong, Zhang Hua, Tai Ping, Mu Xinyi, Zhou Bo, Zhang Meijia, Xia Guoling
机构信息
State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100094, PR China.
出版信息
Front Biosci. 2008 May 1;13:7087-95. doi: 10.2741/3212.
Phosphodiesterase type 5 (PDE5), a cGMP specific, cGMP binding phosphodiesterase, specifically hydrolyzes cGMP to 5'-GMP. Here, we examine the distribution of PDE5 in mouse ovary and its effects on spontaneous maturation of mouse oocytes. PDE5 is present in oocytes and cumulus cells of big, antral follicles. Inhibition of activity of PDE5 significantly and reversibly inhibits spontaneous maturation of cumulus-oocyte complexes (COCs). Suppressive effect of PDE5 on spontaneous maturation of COCs is not blocked by the inhibitor of cGMP-dependent protein kinase (PKG). While Sildenafil, an inhibitor of PDE5, has a poor effect on cGMP levels, it significantly increases cAMP levels. These results suggest that the activity of PDE5 plays a role in regulating spontaneous maturation of mouse oocytes and imply that an interaction between cGMP and cAMP signal is involved in this process.
5型磷酸二酯酶（PDE5）是一种特异性作用于环磷酸鸟苷（cGMP）、能与cGMP结合的磷酸二酯酶，可将cGMP特异性水解为5'-鸟苷单磷酸（5'-GMP）。在此，我们研究了PDE5在小鼠卵巢中的分布及其对小鼠卵母细胞自发成熟的影响。PDE5存在于大型窦状卵泡的卵母细胞和卵丘细胞中。抑制PDE5的活性可显著且可逆地抑制卵丘-卵母细胞复合体（COC）的自发成熟。PDE5对COC自发成熟的抑制作用不会被cGMP依赖性蛋白激酶（PKG）抑制剂所阻断。虽然PDE5抑制剂西地那非对cGMP水平影响不大，但它能显著提高环磷酸腺苷（cAMP）水平。这些结果表明，PDE5的活性在调节小鼠卵母细胞自发成熟过程中发挥作用，提示cGMP与cAMP信号之间的相互作用参与了这一过程。
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