Zhou Tong, Zhang Yanyun, Sun Guizhi, Zou Jie, Li Xiao, Cai Minchao, Xiao Yichuan, Zhang Yumei, Zhao Yapeng, Chen Nan
Department of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Rui jin Er road, Shanghai 200025, China.
Front Biosci. 2008 May 1;13:7269-76. doi: 10.2741/3227.
Dendritic cell (DC)-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a DC-specific C-type lectin that plays an important role in recognizing and capturing pathogens, DC migration and initiation of T cell responses. Here, we show that anti-P-selectin lectin-EGF domain monoclonal antibody (PsL-EGFmAb), originally prepared for blockade of the adhesive molecule P-selectin, significantly down-regulated DC-SIGN expression as well as expression of mature DC-related molecules including CD83, CD86 and CD80 on human DCs. This PsL-EGFmAb treatment of DCs resulted in impaired allogeneic T cell proliferation and IL-12 production. Furthermore, we show that PsL-EGFmAb-induced down-regulation of DC-SIGN may inhibit NF-kappaB expression in DCs, which accounts for the inhibition of DC maturation and stimulatory function. Our present studies indicate that PsL-EGFmAb may be a useful reagent for regulating DC-SIGN expression and DC function.
树突状细胞（DC）特异性细胞间黏附分子3结合非整合素（DC-SIGN）是一种DC特异性C型凝集素，在识别和捕获病原体、DC迁移及T细胞反应的启动中发挥重要作用。在此，我们表明，最初制备用于阻断黏附分子P-选择素的抗P-选择素凝集素-表皮生长因子结构域单克隆抗体（PsL-EGFmAb），可显著下调人DC上DC-SIGN的表达以及包括CD83、CD86和CD80在内的成熟DC相关分子的表达。用这种PsL-EGFmAb处理DC导致异体T细胞增殖和IL-12产生受损。此外，我们表明，PsL-EGFmAb诱导的DC-SIGN下调可能抑制DC中NF-κB的表达，这解释了对DC成熟和刺激功能的抑制。我们目前的研究表明，PsL-EGFmAb可能是调节DC-SIGN表达和DC功能的有用试剂。
