Sytkowski A J, Feldman L, Zurbuch D J
Department of Medicine, New England Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02215.
Biochem Biophys Res Commun. 1991 Apr 30;176(2):698-704. doi: 10.1016/s0006-291x(05)80240-2.
Controversy exists regarding the functional role of N-linked oligosaccharides in the hormone erythropoietin. We have now examined the role of carbohydrates in the hormone's action using quantitative enzymatic deglycosylation. N-deglycosylated hormone exhibited full biological activity and potency in vitro. Denaturing with 6M urea and renaturing revealed that both the native and N-deglycosylated forms recovered full activity as long as the intrachain disulfide bonds remained intact. Therefore, receptor recognition, subsequent biological activity and maintenance of tertiary structure are intrinsic properties of the polypeptide chain of erythropoietin.
关于N-连接寡糖在促红细胞生成素激素中的功能作用存在争议。我们现在使用定量酶促去糖基化方法研究了碳水化合物在该激素作用中的作用。N-去糖基化激素在体外表现出完全的生物学活性和效力。用6M尿素变性并复性后发现，只要链内二硫键保持完整，天然形式和N-去糖基化形式都能恢复完全活性。因此，受体识别、随后的生物学活性和三级结构的维持是促红细胞生成素多肽链的固有特性。
