Gogacz Marek, Bogusiewicz Michał, Putowski Lechosław, Adamiak Aneta, Wertel Iwona, Jakowicki Jerzy A, Rechberger Tomasz
II Katedra i Klinika Ginekologii Akademii Medycznej im. Prof. Feliksa Skubiszewskiego w Lublinie, Lublin.
Ginekol Pol. 2008 Jan;79(1):31-5.
Tumor necrosis factor-alpha (TNF-alpha) plays a key role in the processes underlying the development of pelvic endometriosis. TNF-alpha acts on target cells via two receptors: TNFR1(p55) and TNFR2(p75). Depending on cell type and its activation state, ligand binding to TNF-alpha may induce activation and proliferation of the cells or promote apoptosis. The aim of our study has been to evaluate the expression of TNFR1 and TNFR2 on peritoneal fluid macrophages and T lymphocytes derived from women with endometriosis.
The study group consisted of 22 patients with endometriosis (stage I and II rAFS). 14 patients with benign, non-inflammatory ovarian tumors composed the reference group. Mononuclear cells have been isolated from peritoneal fluid, obtained during laparoscopy. The expression of TNFR1 and TNFR2 proteins has been evaluated by means of flow cytometry, using monoclonal antibodies against CD120a, CD120b, CD3 and CD14.
The percentage of peritoneal fluid macrophages revealing the expression of TNFR1 and TNFR2 proteins has been higher in patients with endometriosis, in comparison with control group (22.6+/-5.3% vs. 6.8+/-1,8%; p=0.03 and 29.3+/-2.3% vs. 8.8+/-1.8%; p=0.01, respectively). The percentage of T lymphocytes with the expression of TNFR1 and TNFR2 has been similar in endometriosis and control group.
Higher percentage of peritoneal fluid macrophages expressing TNFR1 and TNFR2 proteins in endometriosis suggests dependence of these cells on TNF-alpha stimulation. Changes in TNF receptors distribution on PF macrophages, but not lymphocyte, may play its role in the pathogenesis of endometriosis.
肿瘤坏死因子-α（TNF-α）在盆腔子宫内膜异位症发生发展过程中起关键作用。TNF-α通过两种受体作用于靶细胞：TNFR1（p55）和TNFR2（p75）。根据细胞类型及其激活状态，TNF-α与配体结合可能诱导细胞激活和增殖，或促进细胞凋亡。本研究旨在评估子宫内膜异位症患者腹水中巨噬细胞和T淋巴细胞上TNFR1和TNFR2的表达。
研究组由22例子宫内膜异位症患者（rAFS I期和II期）组成。14例患有良性、非炎性卵巢肿瘤的患者作为参照组。单核细胞从腹腔镜检查时获取的腹水中分离得到。使用抗CD120a、CD120b、CD3和CD14的单克隆抗体，通过流式细胞术评估TNFR1和TNFR2蛋白的表达。
与对照组相比，子宫内膜异位症患者腹水中显示TNFR1和TNFR2蛋白表达的巨噬细胞百分比更高（分别为22.6±5.3% 对6.8±1.8%；p = 0.03和29.3±2.3% 对8.8±1.8%；p = 0.01）。子宫内膜异位症组和对照组中表达TNFR1和TNFR2的T淋巴细胞百分比相似。
子宫内膜异位症患者腹水中表达TNFR1和TNFR2蛋白的巨噬细胞百分比更高，提示这些细胞依赖于TNF-α刺激。腹水中巨噬细胞而非淋巴细胞上TNF受体分布的变化可能在子宫内膜异位症的发病机制中起作用。
