Gogacz M, Jakowicki J, Kotarski J
Kliniki Ginekologii Operacyjnej II Katedry Połoznictwa i Chorób Kobiecych Akademii Medycznej w Lublinie.
Ginekol Pol. 2001 May;72(5):402-7.
Phagocytic activity of macrophages isolated from peritoneal fluid (PF) was estimated using flow cytometry. Study group consists of 28 patients with endometriosis and 19 patients with benign noninflammatory tumour of adnex(is) served as reference group. Macrophages were processed in two ways: fresh cells were obtained from women with endometriosis (n = 7) and reference group (n = 10) and frozen cells derived from patients with endometriosis (n = 21) and reference group (n = 9). Phagocytic activity of macrophages was measured against opsonized and conjugated with FITC E. coli. It is worth to notify that phagocytosis was determined in PF environment in the study. Percentage of phagocytosing fresh macrophages did not differ (p = 0.05) between subjected groups of patients and was respectively 64.3% +/- 17.3% vs 49.0 +/- 4.0%. Phagocytic activity of frozen macrophages derived from patients with endometriosis was significantly higher (p < 0.02) in comparison to reference group (14.3 +/- 9.1% vs 5.2 +/- 2.8%).
采用流式细胞术评估从腹腔液（PF）中分离出的巨噬细胞的吞噬活性。研究组由28例子宫内膜异位症患者组成，19例附件良性非炎性肿瘤患者作为参照组。巨噬细胞通过两种方式处理：从子宫内膜异位症患者（n = 7）和参照组（n = 10）获取新鲜细胞，以及从子宫内膜异位症患者（n = 21）和参照组（n = 9）获取冻存细胞。针对经调理素处理并与异硫氰酸荧光素（FITC）结合的大肠杆菌测量巨噬细胞的吞噬活性。值得注意的是，本研究在PF环境中测定吞噬作用。受试患者组之间吞噬新鲜巨噬细胞的百分比无差异（p = 0.05），分别为64.3% ± 17.3%和49.0 ± 4.0%。与参照组相比，子宫内膜异位症患者来源的冻存巨噬细胞的吞噬活性显著更高（p < 0.02）（14.3 ± 9.1%对5.2 ± 2.8%）。
