对戊烷脒耐药的亚马逊利什曼原虫（利什曼原虫属）前鞭毛体的特性研究
Characterization of Leishmania (Leishmania) amazonensis promastigotes resistant to pentamidine.
作者信息
Coelho Adriano C, Gentil Luciana G, da Silveira José Franco, Cotrim Paulo C
机构信息
Instituto de Medicina Tropical, Departamento Moléstias infecciosas e Parasitárias, Faculdade de Medicina da Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470, 4 degrees andar, 05403-000 São Paulo, SP, Brazil.
出版信息
Exp Parasitol. 2008 Sep;120(1):98-102. doi: 10.1016/j.exppara.2008.03.018. Epub 2008 Apr 13.
Pentamidine is a second-line agent used in the treatment of leishmaniasis and its mode of action and mechanism of resistance is not well understood. It was previously demonstrated that transfection of promastigotes and amastigotes with the ABC transporter PRP1 gene confers resistance to pentamidine. To further clarify this point, we generated Leishmania amazonensis mutants resistant to pentamidine. Our results indicated that this ABC transporter is not associated with pentamidine resistance in lines generated by drug pressure through amplification or overexpression mechanisms of PRP1 gene.
喷他脒是用于治疗利什曼病的二线药物，其作用方式和耐药机制尚未完全明确。先前有研究表明，用ABC转运蛋白PRP1基因转染前鞭毛体和无鞭毛体可使其对喷他脒产生耐药性。为进一步阐明这一点，我们构建了对喷他脒耐药的亚马逊利什曼原虫突变体。我们的结果表明，在通过PRP1基因扩增或过表达机制产生的药物压力下，该ABC转运蛋白与喷他脒耐药性无关。
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