Repina Svetlana V, Repin Nikolay V
Department of Cryobiophysics, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Pereyaslavskaya str. 23, Kharkov, Ukraine.
Bioelectrochemistry. 2008 Aug;73(2):106-9. doi: 10.1016/j.bioelechem.2008.04.009. Epub 2008 Apr 12.
The binding of acids and alkalis, formed in tissues by metabolism, along with oxygen and CO(2) transport are recognise as the principal functions of red blood cells (RBCs). Decreases in internal environment pH may result in activation of potential endogenous cytotoxic metabolites, OH and oxidant formation and, as a consequence, result in oxidative damage of cell membrane leading to hemolysis. The characteristics of acid hemolysis in hibernating mammals have been determined in this study. Parameters of HCl-hemolysis, such as the average time for RBC hemolysis and the population distribution of the response, have been investigated. Measurements were performed within 40-5 degrees C temperature range. The resistance of hibernator RBCs to increased acidity, determined according to the acid hemolysis parameters, was found to reflect whether the animal was in the summer active period or in hibernation, with differences also apparent at different points with the hibernation season itself. It was demonstrated that hemolysis parameters of naturally cold-tolerant organisms are altered by decreases in temperature. We discuss a role of cytoskeletal-membrane interactions as a fast-acting switch of the structural and functional state of hibernator RBCs as an adaptation mechanism to acidosis arising from hypothermia and hypermetabolism.
新陈代谢在组织中形成的酸和碱的结合，以及氧气和二氧化碳的运输，被认为是红细胞（RBC）的主要功能。内环境pH值的降低可能会导致潜在的内源性细胞毒性代谢产物、OH和氧化剂的形成，从而导致细胞膜的氧化损伤，进而导致溶血。本研究确定了冬眠哺乳动物酸溶血的特征。研究了HCl溶血的参数，如红细胞溶血的平均时间和反应的群体分布。测量在40至5摄氏度的温度范围内进行。根据酸溶血参数确定的冬眠动物红细胞对酸度增加的抵抗力，被发现反映了动物是处于夏季活跃期还是处于冬眠状态，在冬眠季节本身的不同时间点也存在差异。结果表明，自然耐寒生物的溶血参数会因温度降低而改变。我们讨论了细胞骨架-膜相互作用作为冬眠动物红细胞结构和功能状态的快速作用开关的作用，这是一种对低温和高代谢引起的酸中毒的适应机制。
