Chand Ramesh, Sen Devyani, Prasad K D, Singh A K, Bashyal B M, Prasad L C, Joshi A K
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.
Indian J Exp Biol. 2008 Apr;46(4):249-53.
Screening for resistant barley genotypes in response to fungal toxin of Bipolaris sorokiniana was assessed on standing barley plants as well as in selected callus lines of the same. For the standing lines tested, those manifesting chlorosis in response to toxin infiltration showed a significantly slower disease progress as compared to the necrotic lines. Also, necrosis in the callus tissues of the susceptible cultivar in MS medium supplemented with different concentrations of the crude toxin was significantly higher than in the callus tissues of the chlorotic lines studied. Similar host response to the toxin in in vitro and field situations open up the possibility of screening barley cultivars for resistance to spot blotch using callus culture as against classical methods of screening in order to increase accuracy and save time and space.
针对大麦云纹病菌真菌毒素，对田间大麦植株以及同一植株的选定愈伤组织系进行了抗大麦基因型筛选评估。对于所测试的田间植株系，与坏死型植株相比，毒素浸润后表现出黄化的植株病害进展明显较慢。此外，在添加不同浓度粗毒素的MS培养基中，感病品种愈伤组织的坏死率显著高于所研究的黄化型愈伤组织。在体外和田间条件下，宿主对毒素的类似反应为利用愈伤组织培养筛选大麦品种对叶斑病的抗性开辟了可能性，相对于传统筛选方法，这样可以提高准确性并节省时间和空间。
