McPherson D R, Blankenship J E
Marine Biomedical Institute, University of Texas Medical Branch, Galveston.
Neurosci Lett. 1991 Feb 25;123(2):148-51. doi: 10.1016/0304-3940(91)90917-i.
Injection of rhodamine-conjugated latex microspheres into the right bag cell cluster of Aplysia brasiliana yielded retrograde labelling of a small number of cells in the cerebral and abdominal ganglia. Subsequent staining for serotonin immunoreactivity demonstrated consistent double-labelling in specific cerebral and abdominal ganglion serotonergic cells. The double-labelled populations were also stained in vivo by prior treatment with 5,7-dihydroxytryptamine. These retrogradely labelled serotonergic neurons may represent sources of inhibitory input to the neuroendocrine bag cells.
将罗丹明偶联的乳胶微球注射到巴西耳乌贼的右侧袋状细胞簇中，可对脑和腹神经节中的少量细胞进行逆行标记。随后进行的血清素免疫反应性染色显示，在特定的脑和腹神经节血清素能细胞中存在一致的双重标记。在用5,7 - 二羟基色胺进行预先处理后，这些双重标记的细胞群体在体内也被染色。这些逆行标记的血清素能神经元可能代表了对神经内分泌袋状细胞的抑制性输入来源。
