Lee Je-Jung, Kwak Hee-Jin, Lee Yun-Mi, Lee Joong-Won, Park Myung-Jin, Ko Young-Gyu, Choi Hyung-Do, Kim Nam, Pack Jeong-Ki, Hong Seok-Il, Lee Jae-Seon
Laboratory of Radiation Molecular Oncology, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
Bioelectromagnetics. 2008 Dec;29(8):615-25. doi: 10.1002/bem.20427.
Although in vitro studies have been previously conducted to determine the biological effects of radio frequency (RF) radiation, it has not yet been determined whether or not RF radiation poses a potential hazard. This study was conducted to determine whether RF radiation exposure exerts detectable effects on cell cycle distribution, cellular invasion, and migration. NIH3T3 mouse fibroblasts were exposed to 849 MHz of RF radiation at average SAR values of 2 or 10 W/kg for either 1 h, or for 1 h per day for 3 days. During the exposure period, the temperature in the exposure chamber was maintained isothermally by circulating water throughout the cavity. Cell cycle distribution was analyzed at 24 and 48 h after exposure, by flow cytometry. We detected no statistically significant differences between the sham-exposed and RF radiation-exposed cells. Cellular invasion and migration were assessed by in vitro Matrigel invasion and Transwell migration assays. The RF radiation-exposed groups evidenced no significant changes in motility and invasiveness compared to the sham-exposed group. However, the ionizing radiation-exposed cells, used as a positive control group, manifested dramatic alterations in their cell cycle distribution, cellular invasiveness, and migration characteristics. Our results show that 849 MHz RF radiation exposure exerts no detectable effects on cell cycle distribution, cellular migration, or invasion at average SAR values of 2 or 10 W/kg.
尽管此前已开展体外研究以确定射频（RF）辐射的生物学效应，但尚未确定RF辐射是否构成潜在危害。本研究旨在确定暴露于RF辐射是否会对细胞周期分布、细胞侵袭和迁移产生可检测到的影响。将NIH3T3小鼠成纤维细胞暴露于平均比吸收率（SAR）值为2或10W/kg的849MHz RF辐射下1小时，或每天暴露1小时，共3天。在暴露期间，通过在整个腔体内循环水使暴露室内的温度保持等温。在暴露后24小时和48小时通过流式细胞术分析细胞周期分布。我们未检测到假暴露组和RF辐射暴露组之间存在统计学上的显著差异。通过体外基质胶侵袭和Transwell迁移试验评估细胞侵袭和迁移。与假暴露组相比，RF辐射暴露组在运动性和侵袭性方面未表现出显著变化。然而，用作阳性对照组的电离辐射暴露细胞在其细胞周期分布、细胞侵袭性和迁移特性方面表现出显著改变。我们的结果表明，在平均SAR值为2或10W/kg时，暴露于849MHz RF辐射不会对细胞周期分布、细胞迁移或侵袭产生可检测到的影响。
