Lantow M, Viergutz T, Weiss D G, Simkó M
University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, D-18059, Rostock, Germany.
Radiat Res. 2006 Sep;166(3):539-43. doi: 10.1667/RR3601.1.
The possible harmful effects of radiofrequency electromagnetic fields (RF EMFs) are controversial. We have used human Mono Mac 6 cells to investigate the influence of RF EMFs in vitro on cell cycle alterations and BrdU uptake, as well as the induction of apoptosis and necrosis in human Mono Mac 6 cells, using flow cytometry after exposure to a 1,800 MHz, 2 W/kg specific absorption rate (SAR), GSM-DTX signal for 12 h. No statistically significant differences in the induction of apoptosis or necrosis, cell cycle kinetics, or BrdU uptake were detected after RF EMF exposure compared to sham or incubator controls. However, in the positive control cells treated with gliotoxin and PMA (phorbol 12 myristate-13 acetate), a significant increase in apoptotic and necrotic cells was seen. Cell cycle analysis or BrdU incorporation for 72 h showed no differences between RF EMF- or sham-exposed cells, whereas PMA treatment induced a significant accumulation of cells in G(0)/G(1)-phase and a reduction in S-phase cells. RF EMF radiation did not induce cell cycle alterations or changes in BrdU incorporation or induce apoptosis and necrosis in Mono Mac 6 cells under the exposure conditions used.
射频电磁场（RF EMFs）可能产生的有害影响存在争议。我们使用人单核细胞白血病细胞株Mono Mac 6来研究体外RF EMFs对细胞周期改变和BrdU摄取的影响，以及对人Mono Mac 6细胞凋亡和坏死的诱导作用，在暴露于1800 MHz、2 W/kg比吸收率（SAR）的GSM-DTX信号12小时后采用流式细胞术进行检测。与假照射或培养箱对照相比，RF EMF暴露后在凋亡或坏死诱导、细胞周期动力学或BrdU摄取方面未检测到统计学上的显著差异。然而，在用gliotoxin和PMA（佛波醇12-肉豆蔻酸酯-13-乙酸酯）处理的阳性对照细胞中，凋亡和坏死细胞显著增加。细胞周期分析或72小时的BrdU掺入显示，RF EMF暴露组细胞与假照射组细胞之间没有差异，而PMA处理诱导细胞在G(0)/G(1)期显著积累，S期细胞减少。在所使用的暴露条件下，RF EMF辐射未诱导Mono Mac 6细胞的细胞周期改变、BrdU掺入变化或凋亡和坏死。
