Hillwig Matthew L, Hammer Kimberly D P, Birt Diane F, Wurtele Eve Syrkin
Center for Research on Botanical Dietary Supplements, Department of Genetics, Development, and Cell Biology, and Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011, USA.
J Agric Food Chem. 2008 Jun 25;56(12):4359-66. doi: 10.1021/jf800411v. Epub 2008 May 31.
In this paper we characterize the metabolic fingerprint and first reported anti-inflammatory activity of Hypericum gentianoides. H. gentianoides has a history of medical use by Native Americans, but it has been studied very little for biological activity. High-performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses of a methanol extract show that H. gentianoides contains a family of over nine related compounds that have retention times, mass spectra, and a distinctive UV absorption spectra characteristic of certain acyl-phloroglucinols. These metabolites are abundant relative to other secondary products present in H. gentianoides, accounting for approximately 0.2 g per gram of dry plant tissue. H. gentianoides methanol extracts and a specific semipreparative HPLC fraction from these extracts containing the putative acyl-phloroglucinols reduce prostaglandin E 2 synthesis in mammalian macrophages.
在本文中，我们描述了金丝桃属植物龙胆叶金丝桃的代谢指纹图谱，并首次报道了其抗炎活性。龙胆叶金丝桃有被美洲原住民用于医疗的历史，但对其生物活性的研究很少。对甲醇提取物进行的高效液相色谱（HPLC）和液相色谱 - 电喷雾电离 - 质谱（LC - ESI - MS）分析表明，龙胆叶金丝桃含有一族超过九种相关化合物，这些化合物具有特定酰基间苯三酚的保留时间、质谱和独特的紫外吸收光谱特征。相对于龙胆叶金丝桃中存在的其他次生产物，这些代谢物含量丰富，每克干燥植物组织中约含0.2克。龙胆叶金丝桃甲醇提取物以及这些提取物中含有假定酰基间苯三酚的特定半制备HPLC馏分可降低哺乳动物巨噬细胞中前列腺素E2的合成。
