Fuke Satoshi, Kametani Mizue, Kato Tadafumi
Laboratory for Molecular Dynamics of Mental Disorders, Brain Science Institute, RIKEN, Saitama, Japan.
Neurosci Lett. 2008 Jul 11;439(2):173-7. doi: 10.1016/j.neulet.2008.05.009. Epub 2008 May 8.
Several reports have suggested a role for mitochondrial dysfunction in the pathophysiology of bipolar disorder and schizophrenia. We have focused on the relationship between deleted mitochondrial DNA (mtDNA) and bipolar disorder. To investigate this relationship, we developed a methodology for quantification of the common 4977-bp deletion of mtDNA based on real-time polymerase chain reaction with SYBR Green. In this study, we assessed accumulation of the common deletion in postmortem frontal cortex from 147 individuals (48 controls, 49 patients with bipolar disorder, 50 patients with schizophrenia). We demonstrated age-dependent accumulation of the common deletion of mtDNA (p=1.09E-10). Females showed significantly higher accumulation of the deletion than did males (p=0.002). There was no significant association between accumulation and the two studied major mental disorders in the frontal cortex (p>0.2). However, there was no statistically significant correlation between the common deletion and aging in female patients with bipolar disorder (p=0.133), and no significant sex difference in patients with bipolar disorder (p=0.509). These results indicate that aging and sex have effect on accumulation of the common deletion of mtDNA in the prefrontal cortex depending on the diagnosis.
几份报告表明线粒体功能障碍在双相情感障碍和精神分裂症的病理生理学中起作用。我们专注于缺失的线粒体DNA（mtDNA）与双相情感障碍之间的关系。为了研究这种关系，我们开发了一种基于SYBR Green实时聚合酶链反应定量mtDNA常见4977碱基对缺失的方法。在本研究中，我们评估了147名个体（48名对照、49名双相情感障碍患者、50名精神分裂症患者）死后额叶皮质中常见缺失的积累情况。我们证明了mtDNA常见缺失的积累与年龄相关（p = 1.09E - 10）。女性的缺失积累明显高于男性（p = 0.002）。额叶皮质中缺失积累与两种研究的主要精神障碍之间无显著关联（p>0.2）。然而，双相情感障碍女性患者中常见缺失与衰老之间无统计学显著相关性（p = 0.133），双相情感障碍患者中无显著性别差异（p = 0.509）。这些结果表明，根据诊断情况，衰老和性别对前额叶皮质中mtDNA常见缺失的积累有影响。
