Di Sotto Antonella, Evandri Maria Grazia, Mazzanti Gabriela
Department of Human Physiology and Pharmacology, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Mutat Res. 2008 May 31;653(1-2):130-3. doi: 10.1016/j.mrgentox.2008.04.004. Epub 2008 Apr 22.
The mutagenic and antimutagenic effects of linalool, linalyl acetate and beta-caryophyllene were evaluated by the bacterial reverse mutation assay on Salmonella typhimurium TA 98 and TA 100, and on Escherichia coli WP2uvrA strains. Neither linalool nor beta-caryophyllene showed mutagenicity, but linalyl acetate induced a statistically significant increase in the number of revertant colonies in WP2uvrA, both with and without S9 mixture. Linalool was devoid of antimutagenic activity against 2-nitrofluorene (2NF), sodium azide (SA), methyl methane sulfonate (MMS) and 2-aminoanthracene (2AA). In contrast, beta-caryophyllene showed a strong antimutagenic activity against 2NF: at the maximum concentration tested (6.40mg/plate) the number of 2NF-induced revertant colonies was reduced by 83.9%. beta-Caryophyllene also showed to counteract the mutagenicity of SA (in TA 100), MMS and 2AA (in WP2uvrA): the effect was weak against SA (inhibition lower than 25%) and moderate against MMS and 2AA (up to 30.5%). The antimutagenic activity of beta-caryophyllene observed here suggests further studies to evaluate its possible chemopreventive properties.
通过在鼠伤寒沙门氏菌TA 98和TA 100以及大肠杆菌WP2uvrA菌株上进行细菌回复突变试验，评估了芳樟醇、乙酸芳樟酯和β-石竹烯的致突变和抗突变作用。芳樟醇和β-石竹烯均未显示出致突变性，但乙酸芳樟酯在有和没有S9混合物的情况下，均使WP2uvrA中回复菌落的数量有统计学意义的增加。芳樟醇对2-硝基芴（2NF）、叠氮化钠（SA）、甲基磺酸甲酯（MMS）和2-氨基蒽（2AA）没有抗突变活性。相比之下，β-石竹烯对2NF显示出很强的抗突变活性：在测试的最大浓度（6.40mg/平板）下，2NF诱导的回复菌落数量减少了83.9%。β-石竹烯还显示出能抵消SA（在TA 100中）、MMS和2AA（在WP2uvrA中）的致突变性：对SA的作用较弱（抑制率低于25%），对MMS和2AA的作用中等（高达30.5%）。此处观察到的β-石竹烯的抗突变活性表明需要进一步研究以评估其可能的化学预防特性。
