Department of Biology, Science Faculty, Atatürk University, 25240 Erzurum, Turkey.
Phytother Res. 2010 Jul;24(7):1014-8. doi: 10.1002/ptr.3059.
Medical plants and their various extracts have been occasionally used in the treatment of many diseases. Astragalus is one of those medical plants and it has several biological activities. In the present study, the hexane extracts of six Astragalus species, which are grown in the eastern Anatolia region of Turkey, were isolated, and their mutagenic and antimutagenic properties were investigated by using Salmonella typhimurium TA1535, TA1537 and Escherichia coli WP2uvrA tester strains at 0.05, 0.5 and 5 microg/plate concentrations. Known mutagens sodium azide (NaN(3)), 9-Aminoacridine (9-AA) and N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) were used to determine antimutagenic properties of hexane extracts. The results showed that all hexane extracts, investigated in the present study, can be considered genotoxically safe because they do not have mutagenic activity at the tested concentrations. But, a great many of them have antimutagenic activity against 9-Aminoacridine known as a model intercalator agent. The inhibition rates obtained from the antimutagenicity assays ranged from 27.51% (A. macrocephalus--0.05 microg/plate) to 54.39% (A. galegiformis--5 microg/plate). These activities are valuable toward an extension of the employ of these drugs as new phytotherapeutic or preservative ingredients.
药用植物及其各种提取物偶尔被用于治疗许多疾病。黄芪就是其中一种药用植物，它具有多种生物活性。在本研究中，从生长在土耳其东安纳托利亚地区的六种黄芪物种中分离出了正己烷提取物，并利用鼠伤寒沙门氏菌 TA1535、TA1537 和大肠杆菌 WP2uvrA 测试菌株，在 0.05、0.5 和 5μg/平板浓度下，研究了它们的致突变性和抗突变性。使用已知的诱变剂叠氮化钠（NaN（3））、9-氨基吖啶（9-AA）和 N-甲基-N'-硝基-N-亚硝基胍（MNNG）来确定正己烷提取物的抗突变性。结果表明，本研究中研究的所有正己烷提取物在测试浓度下均没有致突变活性，因此可以认为它们具有遗传毒性安全性。但是，它们中的许多物质对 9-氨基吖啶具有抗突变活性，9-氨基吖啶是一种模型嵌入剂。抗突变性测定中获得的抑制率范围为 27.51%（A. macrocephalus-0.05μg/平板）至 54.39%（A. galegiformis-5μg/平板）。这些活性对于将这些药物作为新的植物治疗或防腐剂成分的应用具有重要意义。
