使用动态原子力显微镜时揭示液体中软生物样品上的成像力：以病毒衣壳为例的研究
Unmasking imaging forces on soft biological samples in liquids when using dynamic atomic force microscopy: a case study on viral capsids.
作者信息
Xu Xin, Carrasco Carolina, de Pablo Pedro Jose, Gomez-Herrero Julio, Raman Arvind
机构信息
School of Mechanical Engineering and Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Biophys J. 2008 Sep;95(5):2520-8. doi: 10.1529/biophysj.108.132829. Epub 2008 May 30.
Abstract
Dynamic atomic force microscopy is widely used for the imaging of soft biological materials in liquid environments; yet very little is known about the peak forces exerted by the oscillating probe tapping on the sample in liquid environments. In this article, we combine theory and experiments in liquid on virus capsids to propose scaling laws for peak interaction forces exerted on soft samples in liquid environments. We demonstrate how these laws can be used to choose probes and operating conditions to minimize imaging forces and thereby robustly image fragile biological samples.
摘要
动态原子力显微镜被广泛用于在液体环境中对柔软生物材料进行成像；然而，对于在液体环境中振荡探针轻敲样品时所施加的峰值力，人们了解甚少。在本文中，我们结合了在液体中对病毒衣壳进行的理论和实验，提出了在液体环境中对柔软样品施加的峰值相互作用力的标度律。我们展示了如何利用这些定律来选择探针和操作条件，以最小化成像力，从而稳健地对脆弱的生物样品进行成像。
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Unmasking imaging forces on soft biological samples in liquids when using dynamic atomic force microscopy: a case study on viral capsids.使用动态原子力显微镜时揭示液体中软生物样品上的成像力：以病毒衣壳为例的研究
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