Molácek Jirí, Treska Vladislav, Kobr Jirí, Certík Bohuslav, Skalický Tomás, Kuntscher Vilém, Krízková Vera
Department of Vascular Surgery, Medical Faculty, University Hospital, Charles University, Pilsen, Czech Republic.
J Vasc Res. 2009;46(1):1-5. doi: 10.1159/000135659. Epub 2008 May 31.
Many studies have been performed in order to model abdominal aortic aneurysm (AAA) in an experimental animal, most commonly in small laboratory animals. In our study, we tried to find the best AAA model in a pig by using various mechanical and enzymatic mechanisms.
Twenty-two pigs were operated on. We combined 3 mechanisms of creating an AAA, using an intraluminal infusion of porcine pancreatic elastase into the abdominal aortic segment, application of plastic cuff below the renal arteries causing turbulent blood flow, and inserting a patch into the longitudinal aortotomy.
We found different results in different groups according to the mechanisms used. In group A, with a combination of the intraluminal elastase infusion and application of a stenosing cuff, AAA developed in all 7 animals (100%). In this group, we also found the largest histological changes in the abdominal aorta samples.
The use of intraluminal pancreatic elastase infusion, together with increased turbulent flow caused by the stenosing cuff, seems to be the best model of AAA in pigs. This model is suitable for further research in the etiopathology of AAA. In fact, it is the first successful approach to a large-caliber native aneurysm model.
为了在实验动物中构建腹主动脉瘤（AAA）模型，已经开展了许多研究，最常见的是在小型实验动物中进行。在我们的研究中，我们试图通过使用各种机械和酶促机制在猪身上找到最佳的AAA模型。
对22头猪进行手术。我们结合了3种创建AAA的机制，即将猪胰弹性蛋白酶腔内注入腹主动脉段、在肾动脉下方应用塑料套囊以引起血流紊乱以及在主动脉纵行切开处插入补片。
根据所使用的机制，我们在不同组中发现了不同的结果。在A组中，联合腔内弹性蛋白酶注入和应用狭窄套囊，所有7只动物（100%）均发生了AAA。在该组中，我们还在腹主动脉样本中发现了最大的组织学变化。
腔内注入胰弹性蛋白酶并结合狭窄套囊引起的血流紊乱增加，似乎是猪AAA的最佳模型。该模型适用于AAA病因病理学的进一步研究。事实上，这是构建大口径天然动脉瘤模型的首个成功方法。
