Tucci Junior Silvio, Carvalho Roberto Marins de, Celini Fábia Martins, Cologna Adauto José, Suaid Haylton Jorge, Tirapelli Luis Fernando, Martins Antonio Carlos Pereira
Division of Urology, Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil.
Acta Cir Bras. 2008;23 Suppl 1:42-6; discussion 46. doi: 10.1590/s0102-86502008000700008.
To evaluate the influence of chlorpromazine (CPZ) on renal function and lipid peroxidation in a rat model of kidney ischemia/reperfusion injury.
Forty eight Wistar rats underwent a laparotomy for hilar clamping of left kidney with a bulldog clamp for 60 minutes followed by organ reperfusion and contralateral nephrectomy. Of these, 26 received 3mg/kg of CPZ intravenously 15 minutes before renal ischemia (G-E) while the remaining 22 were used as ischemic control group (G-C). Eleven rats of G-E and 8 of G-C were followed for blood urea nitrogen and creatinine determinations before renal ischemia and at 1st, 4th and 7th postoperative days. Samplings of left renal tissue were obtained at 5 minutes (5 rats from each group) and 24 hours (9 G-C and 10 of G-E) of reperfusion for malondialdehy (MDA) content determination. Controls of renal MDA content were determined in kidneys harvested from 6 additional normal rats.
Acute renal failure occurred in all animals but levels of BUN and creatinine were significantly lower in G-E (p<0.001). MDA content rose strikingly at 5 minutes of reperfusion in both groups (p>0.05) and returned near to normal levels 24 hours later.
CPZ conferred partial protection of renal function to kidneys submitted to ischemia/reperfusion injury that seems to be not dependent on inhibition of lipid peroxidation.
在大鼠肾脏缺血/再灌注损伤模型中评估氯丙嗪（CPZ）对肾功能和脂质过氧化的影响。
48只Wistar大鼠接受剖腹手术，用牛头夹将左肾肾门夹闭60分钟，随后进行器官再灌注和对侧肾切除术。其中，26只在肾脏缺血前15分钟静脉注射3mg/kg的CPZ（G-E组），其余22只作为缺血对照组（G-C组）。G-E组的11只大鼠和G-C组的8只大鼠在肾脏缺血前以及术后第1、4和7天进行血尿素氮和肌酐测定。在再灌注5分钟（每组5只大鼠）和24小时（G-C组9只，G-E组10只）时获取左肾组织样本，用于测定丙二醛（MDA）含量。另外从6只正常大鼠获取的肾脏中测定肾脏MDA含量作为对照。
所有动物均发生急性肾衰竭，但G-E组的血尿素氮和肌酐水平显著较低（p<0.001）。两组在再灌注5分钟时MDA含量均显著升高（p>0.05），24小时后恢复至接近正常水平。
CPZ对遭受缺血/再灌注损伤的肾脏具有部分肾功能保护作用，这似乎不依赖于脂质过氧化的抑制。
