猿猴病毒40永生化的人成纤维细胞中病毒整合位点的克隆与测序
Cloning and sequencing of viral integration site in human fibroblasts immortalized by simian virus 40.
作者信息
Yano O, Hirano H, Karasaki Y, Higashi K, Nakamura H, Akiya S, Gotoh S
机构信息
Department of Biochemistry, University of Occupational and Environmental Health, Kitakyushu City, Japan.
出版信息
Cell Struct Funct. 1991 Feb;16(1):63-71. doi: 10.1247/csf.16.63.
We have analyzed cellular DNA sequences at the viral genome integration site in a human fibroblast cell line VA13 immortalized by simian virus 40 (SV40). The computer analysis of the junctional cellular DNA sequences did not show any homology to the DNA sequences previously reported. This suggests that immortalization by SV40 was not induced by the destruction of any known oncogene or anti-oncogene at the integration site. We did not find the precise substantial sequence homology at the junctional site between the cellular DNA and SV40 DNA, indicating that the recombination mechanism involved does not require precise sequence homology and therefore, SV40 genome was probably not integrated by homologous recombination. Short direct and inverted repeats of 5 to 29 nucleotides were found in the junctional cellular and SV40 DNA. Cellular DNA abutting SV40 DNA was found by the Northern blot analysis to be expressed in diploid human fibroblasts and SV40-transformed cells. The nature of this RNA is now under study.
我们分析了猿猴病毒40（SV40）永生化的人成纤维细胞系VA13中病毒基因组整合位点处的细胞DNA序列。对连接部位细胞DNA序列的计算机分析未显示与先前报道的DNA序列有任何同源性。这表明SV40介导的永生化不是由整合位点处任何已知癌基因或抗癌基因的破坏所诱导的。我们在细胞DNA与SV40 DNA的连接位点未发现精确的大量序列同源性，这表明所涉及的重组机制不需要精确的序列同源性，因此，SV40基因组可能不是通过同源重组整合的。在连接部位的细胞和SV40 DNA中发现了5至29个核苷酸的短直接重复序列和反向重复序列。通过Northern印迹分析发现，与SV40 DNA相邻的细胞DNA在二倍体人成纤维细胞和SV40转化细胞中表达。这种RNA的性质目前正在研究中。
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