Shimojo Naoki, Katsuki Toshiyuki, Tateno Noriko, Tomiita Minako, Kohno Yoichi
Department of Pediatrics, Graduate School of Medicine, Chiba University, Chiba, Japan.
Int Arch Allergy Immunol. 2008;147(2):110-6. doi: 10.1159/000135697. Epub 2008 Jun 3.
Respiratory syncytial virus (RSV) G protein is involved in Th2-shifted immune response, while F protein has a reverse effect on RSV infection in Th2-prone BALB/c mice. Studies on the human T cell response to F or G protein are few, and the relationship between the immune response to G protein and atopy is not known.
We established CD4+ RSV-specific T cell lines (TCLs) from adult patients with respiratory allergic diseases (allergics) or nonatopic controls (controls), and examined proliferative responses and gamma-interferon (IFN-gamma) and interleukin 4 (IL-4) production in these TCLs upon stimulation with RSV, F or G proteins.
32 and 29 RSV-specific oligoclonal TCLs were established from allergics and controls, respectively. IL-4/IFN-gamma in the culture supernatant of antigen-stimulated TCLs was significantly higher in allergics than in controls (p = 0.042). IL-4/IFN-gamma ratios in the culture supernatants of G-protein-reactive TCLs were significantly higher in allergics than in controls (p = 0.016), while no differences in IL-4/IFN-gamma in culture supernatants of F-protein-reactive TCLs were found between allergics and controls (p = 0.787). IL-4/IFN-gamma in the culture supernatants of G-protein-reactive TCLs was significantly higher than those of F-protein-reactive TCLs in allergics (p = 0.023) but not in controls (p = 0.768).
The results suggest that the T cell response to RSV is influenced by the atopic diathesis as well as by individual RSV antigens, and that G protein may be an important antigen involved in allergy in humans.
呼吸道合胞病毒（RSV）G蛋白参与Th2偏移的免疫反应，而F蛋白对Th2倾向的BALB/c小鼠的RSV感染具有相反作用。关于人类T细胞对F或G蛋白反应的研究较少，且对G蛋白的免疫反应与特应性之间的关系尚不清楚。
我们从患有呼吸道过敏性疾病的成年患者（过敏者）或非特应性对照（对照者）中建立了CD4+ RSV特异性T细胞系（TCLs），并检测了这些TCLs在用RSV、F或G蛋白刺激后的增殖反应以及γ干扰素（IFN-γ）和白细胞介素4（IL-4）的产生。
分别从过敏者和对照者中建立了32个和29个RSV特异性寡克隆TCLs。抗原刺激的TCLs培养上清液中的IL-4/IFN-γ在过敏者中显著高于对照者（p = 0.042）。G蛋白反应性TCLs培养上清液中的IL-4/IFN-γ比值在过敏者中显著高于对照者（p = 0.016），而F蛋白反应性TCLs培养上清液中的IL-4/IFN-γ在过敏者和对照者之间未发现差异（p = 0.787）。G蛋白反应性TCLs培养上清液中的IL-4/IFN-γ在过敏者中显著高于F蛋白反应性TCLs（p = 0.023），但在对照者中无差异（p = 0.768）。
结果表明，T细胞对RSV的反应受特应性素质以及单个RSV抗原的影响，并且G蛋白可能是人类过敏反应中涉及的重要抗原。
