Welén K, Jennbacken K, Tesan T, Damber J-E
Department of Urology, Lundberg Laboratory for Cancer Research, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
Prostate Cancer Prostatic Dis. 2009;12(1):41-6. doi: 10.1038/pcan.2008.33. Epub 2008 Jun 3.
Androgen-independent prostate cancer is an aggressive disease with high angiogenic and metastatic potential. Increased microvessel density and altered invasion properties have previously been described in LNCaP-19, an androgen-independent subline to LNCaP. To characterize the differences in angiogenesis and invasion, the vessels of these tumour xenografts were investigated with immunohistochemistry, and the influence of tumour cells on endothelial cell migration, proliferation and tube formation was studied in vitro. The blood vessels of LNCaP were found to be stabilized by pericytes more frequently than vessels in LNCaP-19. Further, tumour cell invasion was decreased in pericyte-covered blood vessels in both the tumour types. LNCaP-19 displayed an increased potential to induce endothelial cell migration in vitro. In conclusion, pericyte coverage seems to be important for the invasion of tumour cells into blood vessels. Further, LNCaP-19 has lower pericyte coverage and an increased potential to induce endothelial cell migration, which reflects its high microvessel density.
雄激素非依赖性前列腺癌是一种具有高血管生成和转移潜能的侵袭性疾病。先前在LNCaP - 19（LNCaP的一种雄激素非依赖性亚系）中已描述了微血管密度增加和侵袭特性改变。为了表征血管生成和侵袭的差异，用免疫组织化学方法研究了这些肿瘤异种移植的血管，并在体外研究了肿瘤细胞对内皮细胞迁移、增殖和管形成的影响。发现LNCaP的血管比LNCaP - 19中的血管更频繁地被周细胞稳定。此外，两种肿瘤类型中，周细胞覆盖的血管中肿瘤细胞侵袭均减少。LNCaP - 19在体外诱导内皮细胞迁移的潜能增加。总之，周细胞覆盖似乎对肿瘤细胞侵入血管很重要。此外，LNCaP - 19的周细胞覆盖较低且诱导内皮细胞迁移的潜能增加，这反映了其高微血管密度。
