Orr Winson, Molnar Stephen J
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, 960 Carling Avenue, Ottawa, ON K1A0C6, Canada.
Genome. 2008 Jun;51(6):421-5. doi: 10.1139/G08-026.
A key breeding objective in oat (Avena sativa L.) is cultivars with high and low beta-glucan content. In a targeted strategy to develop PCR-based markers linked to published beta-glucan content quantitative trait loci (QTLs) regions, 15 random amplified polymorphic DNA (RAPD) fragments were cloned and their sequences used to design sequence-characterized amplified region (SCAR) and cleaved amplified polymorphic sequence (CAPS) primers. The 13 derived SCAR markers and 2 derived CAPS markers were mapped on either the 'Kanota' x 'Ogle' (KO) or the 'Terra' x 'Marion' (TM) oat reference map. In addition, 3 previously reported SCAR markers were characterized further. Ten SCAR markers and one CAPS marker were associated with beta-glucan QTL regions and many of these are also associated with QTLs for protein content or other traits. These markers have the potential to help define homologous and homologous relationships in oat and investigate the complex genetics of beta-glucan and protein content.
燕麦（Avena sativa L.）育种的一个关键目标是培育出β-葡聚糖含量高和低的品种。在一项有针对性的策略中，为了开发与已发表的β-葡聚糖含量数量性状位点（QTL）区域相关的基于PCR的标记，克隆了15个随机扩增多态性DNA（RAPD）片段，并利用其序列设计序列特异性扩增区域（SCAR）和酶切扩增多态性序列（CAPS）引物。将13个衍生的SCAR标记和2个衍生的CAPS标记定位在“卡诺塔”ד奥格尔”（KO）或“特拉”ד马里恩”（TM）燕麦参考图谱上。此外，对3个先前报道的SCAR标记进行了进一步鉴定。10个SCAR标记和1个CAPS标记与β-葡聚糖QTL区域相关，其中许多标记也与蛋白质含量或其他性状的QTL相关。这些标记有可能有助于确定燕麦中的同源和同系关系，并研究β-葡聚糖和蛋白质含量的复杂遗传学。
