Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, 960 Carling Ave., Ottawa, ON, K1A 0C6, Canada.
Theor Appl Genet. 2012 Apr;124(6):1115-25. doi: 10.1007/s00122-011-1773-7. Epub 2012 Feb 4.
Seven pairs of oat near-isogenic lines (NILs) (Kibite in Crop Sci 41:277-278, 2001) contrasting for the Dw6 dwarfing gene were used to test for correlation between tall/dwarf phenotype and polymorphic genotype using restriction fragment length polymorphism (RFLP) and other molecular markers selected from the Kanota × Ogle (K×O) (Wight et al. in Genome 46:28-47, 2003) and Terra × Marion (De Koeyer et al. in Theor Appl Genet 108:1285-1298, 2004) recombination maps. This strategy located the Dw6/dw6 locus to a small chromosomal region on K×O linkage group (LG) KO33, near or at a putative RFLP locus aco245z. Aco245z and other tightly linked flanking markers have potential for use in marker-assisted selection (MAS), and PCR-based markers were developed from several of these. RFLP genotyping of the Dw6 NILs indicated that 13 of the 14 individual lines were homogeneously maternal or paternal for a large genomic region near Dw6/dw6, an unexpected result for NILs. The cDNA clone aco245 codes for a vacuolar proton ATPase subunit H, a potential candidate gene for Dw6. Vacuolar proton ATPase enzymes have a central role in plant growth and development and a mutation in subunit C is responsible for the det3 dwarfing mutation in Arabidopsis thaliana (Schumacher et al. in Genes Dev 13:3259-3270, 1999). Aco245 affords the potential of designing highly precise diagnostic markers for MAS for Dw6. The Dw6 NILs have potential utility to investigate the role of vacuolar proton ATPases in growth and development in plants.
利用 7 对 oat 近等基因系（NILs）（Kibite 在 Crop Sci 41:277-278, 2001）进行了 dwarfing 基因 Dw6 的对比试验，这些 NILs 是利用限制片段长度多态性（RFLP）和从 Kanota × Ogle（K×O）（Wight 等人在 Genome 46:28-47, 2003）和 Terra × Marion（De Koeyer 等人在 Theor Appl Genet 108:1285-1298, 2004）重组图谱中选择的其他分子标记对高/矮表型和多态基因型进行相关性分析。这一策略将 Dw6/dw6 基因座定位到 K×O 连锁群（LG）KO33 上的一个小染色体区域，靠近或在一个假定的 RFLP 位点 aco245z 附近。aco245z 和其他紧密连锁的侧翼标记具有用于辅助选择（MAS）的潜力，并且从其中的几个标记开发了基于 PCR 的标记。对 Dw6 NILs 的 RFLP 基因分型表明，14 个个体系中的 13 个在 Dw6/dw6 附近的一个大基因组区域上同质母系或父系，这是 NILs 的一个意外结果。cDNA 克隆 aco245 编码液泡质子 ATP 酶亚基 H，这是 Dw6 的一个潜在候选基因。液泡质子 ATP 酶在植物生长发育中起着核心作用，亚基 C 的突变导致拟南芥 det3 矮化突变（Schumacher 等人在 Genes Dev 13:3259-3270, 1999）。aco245 为 Dw6 的 MAS 设计高度精确的诊断标记提供了潜力。这些 Dw6 NILs 具有潜在的应用价值，可以研究液泡质子 ATP 酶在植物生长发育中的作用。
