墨西哥利什曼原虫M379中的染色体重排

Chromosome rearrangement in Leishmania mexicana M379.

作者信息

Liu J H, Gajendran N, Muthui D, Muyldermans S, Dujardin J C, De Doncker S, Jacquet D, Le Ray D, Mathieu-Daudé F, Hamers R

机构信息

Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.

出版信息

Mol Biochem Parasitol. 1991 May;46(1):53-60. doi: 10.1016/0166-6851(91)90198-f.

DOI:10.1016/0166-6851(91)90198-f

PMID:1852176

Abstract

Circular extrachromosomal elements were observed in a variety of Leishmania species. We show here that two lines originating from the same isolate have been found to contain a circular DNA molecule of 26.6 kb and a linear chromosome of about 250 kb, respectively, which share a homology of more than 20 kb. The circular DNA molecule and its related region on the linear chromosome were cloned and their restriction maps compared. This investigation reveals information about chromosome rearrangement in L. mexicana M379. Further examination will enable us to understand the nature of chromosome rearrangement such as circularization or linearization.

摘要

在多种利什曼原虫物种中观察到环状染色体外元件。我们在此表明，源自同一分离株的两条品系分别被发现含有一个26.6 kb的环状DNA分子和一条约250 kb的线性染色体，它们共享超过20 kb的同源性。对环状DNA分子及其在线性染色体上的相关区域进行了克隆，并比较了它们的限制性图谱。这项研究揭示了墨西哥利什曼原虫M379中染色体重排的信息。进一步的研究将使我们能够了解染色体重排的本质，如环化或线性化。