Tsurumoto Toshiyuki, Zhu Dan, Sommer Andrei P
Department of Orthopaedics, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1, Sakamoto, Nagasaki 852-8501, Japan.
Environ Sci Technol. 2008 May 1;42(9):3324-8. doi: 10.1021/es702857s.
Earlier we introduced a biosensor for the identification of nanobacteria in water drops. Here, we generalize its principle and apply it to identify nanobacteria in synovial fluid from a patient with osteoarthritis. Results indicate the prevalence of nanobacteria in the synovial fluid. The identification method is applicable to body fluids such as unfiltered human blood and urine, is independent of culturing procedures, and permits for a rapid detection of nanoparticles in liquid drops. In view of increasing clinical evidence on a contribution of nanobacteria in disease, their reported detection in HIV-infected people in South Africa, laboratory experiments indicating the excretion of viable (i.e., propagating) nanobacteria from humans via urine, the use of human excreta in agricultural irrigation, models predicting an injection of nanoaerosols contained in irrigation water enriched with human excreta into the atmosphere, and the identification of nanobacteria in the terrestrial atmosphere, promote the identification method described in this work to an important tool to monitor nanobacteria in body fluids and environmental samples.
早些时候，我们介绍了一种用于识别水滴中纳米细菌的生物传感器。在此，我们推广其原理并将其应用于识别骨关节炎患者滑液中的纳米细菌。结果表明滑液中存在纳米细菌。该识别方法适用于未过滤的人体血液和尿液等体液，独立于培养程序，并允许快速检测液滴中的纳米颗粒。鉴于越来越多的临床证据表明纳米细菌在疾病中的作用，在南非艾滋病毒感染者中报告检测到纳米细菌，实验室实验表明人类通过尿液排出有活力（即繁殖）的纳米细菌，在农业灌溉中使用人类排泄物，模型预测富含人类排泄物的灌溉水中所含纳米气溶胶会注入大气，以及在陆地大气中识别纳米细菌，促使将本工作中描述的识别方法提升为监测体液和环境样本中纳米细菌的重要工具。
