Baulina O I, Titel K, Gorelova O A, Malaĭ O V, Ehwald R
Mikrobiologiia. 2008 Mar-Apr;77(2):232-40.
The space of diffusive distribution of neutral hydrophilic macromolecules (dextrans with molecular sizes of 1.5 to 9 nm in the Stokes radius values) in the mucous surface structures (MSS) of intact bacterial cells has been studied for the first time on cyanobacteria. Cyanobacterial species and strains under study belong to different taxonomic groups, the members of which form MSS of various morphology and ultrastructure and can grow in association with plants and animals, inter alia as mucous microcolonies. The range of permeability has been determined by the fractionation of polydisperse dextrans method, previously applied for plants, in combination with electron microscopy. Dextrans are supposedly distributed in the MSS polysaccharide matrix in accordance with their sizes, in much the same way as in a macroporous unitary gel. The similarity of the chemical composition and macromolecular organization of cyanobacterial MSS with pectins of plant cell walls and the role of MSS and the intercellular matrix as permeability barriers in associative interactions of microorganisms are under consideration.
首次在蓝细菌上研究了中性亲水性大分子(斯托克斯半径值为1.5至9纳米的葡聚糖)在完整细菌细胞黏液表面结构(MSS)中的扩散分布空间。所研究的蓝细菌物种和菌株属于不同的分类群,其成员形成了各种形态和超微结构的MSS,并且可以与植物和动物共生生长,尤其是形成黏液微菌落。渗透率范围是通过先前应用于植物的多分散葡聚糖分级分离方法结合电子显微镜测定的。据推测,葡聚糖根据其大小分布在MSS多糖基质中,这与在大孔单一凝胶中的情况非常相似。正在考虑蓝细菌MSS的化学成分和大分子组织与植物细胞壁果胶的相似性,以及MSS和细胞间基质作为微生物缔合相互作用中渗透屏障的作用。