Lee Ki Yong, Kim Seung Hyun, Sung Sang Hyun, Kim Young Choong
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Planta Med. 2008 Jun;74(8):867-9. doi: 10.1055/s-2008-1074552. Epub 2008 Jun 3.
A methanolic extract of the fruits of AMOMUM TSAO-KO (Zingiberaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia. Two new bicyclic nonanes characterized as 6,7-dihydroxy-indan-4-carbaldehyde ( 1) and 6-hydroxy-indan-4-carbaldehyde ( 2) were isolated with the eleven known compounds 6,7-dihydroxy-3,7-dimethyloct-2-enoic acid ( 3), tsaokoin ( 4), isotsaokoin ( 5), 8-oxogeraniol ( 6), P-menth-1-ene-5,6-diol ( 7), 3alpha-hydroxycarvotagenone ( 8), tsaokoarylone ( 9), 1,7-bis(4-hydroxy-3-methoxyphenyl)-4,6-heptadien-3one ( 10), (+)-hannokinol ( 11), MESO-hannokinol ( 12) and hannokinin ( 13), from the fruits of A. TSAO-KO using bioactivity-guided fractionation. All thirteen compounds significantly inhibited lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 1 microM to 100 microM.
草果（姜科）果实的甲醇提取物显著减弱了脂多糖刺激的BV2小胶质细胞中一氧化氮的产生。通过生物活性导向分离法，从草果果实中分离出两种新的双环壬烷，分别鉴定为6,7 - 二羟基茚满-4 - 甲醛（1）和6 - 羟基茚满-4 - 甲醛（2），以及十一种已知化合物，即6,7 - 二羟基-3,7 - 二甲基辛-2 - 烯酸（3）、草果酮（4）、异草果酮（5）、8 - 氧代香叶醇（6）、对薄荷-1 - 烯-5,6 - 二醇（7）、3α - 羟基香芹酮（8）、草果芳酮（9）、1,7 - 双（4 - 羟基-3 - 甲氧基苯基）-4,6 - 庚二烯-3 - 酮（10）、（+）-汉诺醇（11）、内消旋-汉诺醇（12）和汉诺金宁（13）。所有这十三种化合物在1微摩尔至100微摩尔的浓度范围内，均显著抑制脂多糖诱导的BV2小胶质细胞中一氧化氮的产生。
