College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Planta Med. 2010 Jul;76(10):1007-10. doi: 10.1055/s-0029-1240911. Epub 2010 Mar 1.
A methanolic extract of ALNUS FIRMA barks (Betulaceae) significantly attenuated nitric oxide production in lipopolysaccharide-stimulated BV2 microglia. Two new compounds, characterized as 4-hydroxy-2,6-dimethoxyphenyl 6'- O-syringoyl- beta- D-glucopyranoside (1) and 4-hydroxy-2,6-dimethoxyphenyl 6'- O-vanilloyl- beta- D-glucopyranoside (2), were isolated from the barks of A. FIRMA using bioactivity-guided fractionation, together with two known phenolic glycosides (3, 4) and 11 known diarylheptanoids (5- 15). Among these compounds, 1- 4 and 6- 11 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 10 microM to 100 microM.
水曲柳树皮（桦木科）的甲醇提取物能显著抑制脂多糖刺激的 BV2 小胶质细胞中一氧化氮的产生。通过活性导向分离，从水曲柳树皮中分离得到了两个新化合物，分别鉴定为 4-羟基-2,6-二甲氧基苯基 6'-O-对羟基苯甲酰基-β-D-吡喃葡萄糖苷（1）和 4-羟基-2,6-二甲氧基苯基 6'-O-香草酰基-β-D-吡喃葡萄糖苷（2），此外还分离得到了两个已知的酚糖苷（3、4）和 11 个已知的二芳基庚烷类化合物（5-15）。这些化合物中，1-4 和 6-11 在 10μM 至 100μM 的浓度范围内对 LPS 诱导的 BV2 小胶质细胞中一氧化氮的产生有显著的抑制作用。
