[差异泰氏蝎毒液对整体凝血试验的影响。初步研究]

[Effect of Tityus discrepans scorpion venom on global coagulation test. Preliminary studies].

作者信息

Brazón Josmary, Guerrero Belsy, Arocha-Piñango Carmen Luisa, Sevcik Carlos, D'Suze Gina

机构信息

Laboratorio de Neurofarmacología Celular, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 21827, Caracas 1020-A, Venezuela.

出版信息

Invest Clin. 2008 Mar;49(1):49-58.

PMID:18524331

Abstract

Tityus discrepans (Td) scorpion venom modifies clotting times in humans. We studied the in vitro venom effect on partial thromboplastin time (PTT), prothrombin time (PT) and its direct clotting activity, using fresh human plasma and purified fibrinogen as substrates. Whole venom (WV) was fractioned with a Protein Pak 125 molecular exclusion column (0.5 mL/min, CH3COONH4 20 mM, pH 4.7). Six fractions (F1 through F6) with retention times ranging from 12.8 to 31 min were obtained. WV (78 to 625 microg/mL) and fraction F1 (10 to 42.5 microg/mL), shortened PTT; WV (700 to 1000 microg/mL) and fraction F6 (16.5 to 700 microg/mL), prolonged PTT. WV (40 to 240 microg/mL) and fraction F2 (5 to 40 microg/mL), prolonged PT. No thrombin-like activity was found with this venom on human plasma or purified fibrinogen. Td venom contains procoagulant components, able to shorten PTT. In addition, the venom contains anticoagulant components which prolong PT and PTT.

摘要

差异肥须蛛（Td）蝎毒可改变人类的凝血时间。我们使用新鲜人血浆和纯化纤维蛋白原作为底物，研究了该毒液在体外对部分凝血活酶时间（PTT）、凝血酶原时间（PT）及其直接凝血活性的影响。用Protein Pak 125分子排阻柱（0.5 mL/分钟，20 mM醋酸铵，pH 4.7）对全毒液（WV）进行分级分离。获得了六个保留时间在12.8至31分钟之间的级分（F1至F6）。WV（78至625微克/毫升）和级分F1（10至42.5微克/毫升）可缩短PTT；WV（700至1000微克/毫升）和级分F6（16.5至700微克/毫升）可延长PTT。WV（40至240微克/毫升）和级分F2（5至40微克/毫升）可延长PT。该毒液对人血浆或纯化纤维蛋白原未发现类凝血酶活性。Td毒液含有促凝成分，能够缩短PTT。此外，该毒液还含有抗凝成分，可延长PT和PTT。