Björkman A, Lebbad M, Perlmann H, Freeman T, Hogh B, Petersen E, Gille E, Lindgren A, Willcox M, Hanson A P
Yekepa Research Unit, Liberian Institute of Biomedical Research.
Parasite Immunol. 1991 May;13(3):301-11. doi: 10.1111/j.1365-3024.1991.tb00284.x.
The seroreactivities to Pf155/RESA antigen and to three oligopeptides (EENV)2, EENVEHDA and K(DDEHVEEPTVA)2, which constitute repeat subunits of the RESA molecule, were investigated between 1980 and 1986 in two cohorts of children (n = 114) with and without monthly chemosuppression (pulsed reduction of parasite load) against malaria from six months to five years of age during development of protective immunity. Serum samples were collected first at half-yearly and then yearly intervals. Positive immunofluorescence against Pf155/RESA (EMIF) was only found in 24% of the samples. The children with chemosuppression were more often seropositive (30%) than the non prophylactic children (17%). This was in contrast to the seroreactivity against crude parasitic antigens which was highest in the non prophylactic children. In these children, there was a general decrease of EMIF titres around two years of age. Immunosuppression by chronic parasitaemia may be suggested as a reason for this. ELISA seroreactivity was found against one, two or three oligopeptides in all children with high EMIF titres (greater than 250) although (EENV)2 appeared to best correlate (92%) with the EMIF seropositivity. While EMIF seropositivity only showed partial correlation to immunoprotection against patent parasitaemia in the non prophylactic children, the individual profiles of the seroreactivities to the different specific epitopes of the Pf155/RESA molecule and their relevance with regards to protective immunity to malaria need to be investigated further.
1980年至1986年期间，在两组年龄从6个月至5岁、处于保护性免疫形成期的儿童（n = 114）中，研究了他们对Pf155/RESA抗原以及对构成RESA分子重复亚基的三种寡肽（EENV）2、EENVEHDA和K（DDEHVEEPTVA）2的血清反应性。血清样本最初每半年采集一次，之后每年采集一次。仅在24%的样本中发现针对Pf155/RESA（EMIF）的阳性免疫荧光。接受化学抑制治疗的儿童血清反应阳性率（30%）高于未接受预防治疗的儿童（17%）。这与针对粗制寄生虫抗原的血清反应性形成对比，后者在未接受预防治疗的儿童中最高。在这些儿童中，大约两岁时EMIF滴度普遍下降。慢性寄生虫血症导致的免疫抑制可能是其原因。在所有EMIF滴度较高（大于250）的儿童中均发现了针对一种、两种或三种寡肽的ELISA血清反应性，尽管（EENV）2似乎与EMIF血清反应阳性的相关性最佳（92%）。虽然在未接受预防治疗的儿童中，EMIF血清反应阳性仅与针对显性寄生虫血症的免疫保护呈部分相关性，但Pf155/RESA分子不同特异性表位的血清反应性个体谱及其与疟疾保护性免疫的相关性仍需进一步研究。
