Björkman A, Perlmann H, Petersen E, Hogh B, Lebbad M, Warsame M, Hanson A P, Perlmann P
Yekepa Research Unit, Liberian Institute of Biomedical Research.
Parasite Immunol. 1990 Mar;12(2):115-23. doi: 10.1111/j.1365-3024.1990.tb00941.x.
Sera from 32 adult men residing in a malaria holoendemic area of Liberia were investigated for seroreactivities to different asexual blood-stage malaria antigens on five consecutive occasions from 1984 to 1986. The seroreactivities to crude parasitic antigens and to Pf 155/RESA (EMIF) were determined by immunofluorescence and to repetitive sequences of Pf 155/RESA by enzyme-linked immunosorbent assay (ELISA). All sera were highly reactive against the crude parasitic antigens with reciprocal titres varying from 5000 to 100,000. The EMIF titres showed a wider variation from negative (less than 10) to 25,000, and when the same individuals were re-examined on subsequent surveys similar EMIF titres were found. The ELISA seroreactivities to three different repetitive sequences of Pf 155/RESA also showed different individual profiles which were rather consistent on consecutive surveys. High EMIF titres appeared to be correlated mainly to one of the peptide sequences, namely (EENV)2. The consistent individual profiles of the seroreactivities to Pf 155 and its repetitive sequences suggest genetic restriction of the humoral immune response. Although no significant correlation was found between EMIF titres and parasitic densities in the adult hyperimmune men the specific peptides, however, offer new possibilities of further investigating protective capacities of different immune responses to specific epitopes of the malaria parasite.
1984年至1986年期间，对居住在利比里亚疟疾高度流行地区的32名成年男性的血清进行了连续五次调查，以研究其对不同无性血液期疟疾抗原的血清反应性。通过免疫荧光法测定对粗制寄生虫抗原和Pf 155/RESA（EMIF）的血清反应性，通过酶联免疫吸附测定（ELISA）测定对Pf 155/RESA重复序列的血清反应性。所有血清对粗制寄生虫抗原均具有高反应性，抗体效价倒数从5000到100,000不等。EMIF效价变化范围更大，从阴性（小于10）到25,000，并且在后续调查中对同一受试者重新检测时发现了相似的EMIF效价。对Pf 155/RESA三个不同重复序列的ELISA血清反应性也显示出不同的个体特征，这些特征在连续调查中相当一致。高EMIF效价似乎主要与其中一个肽序列即（EENV）2相关。对Pf 155及其重复序列的血清反应性的一致个体特征表明体液免疫反应存在基因限制。尽管在成年超免疫男性中未发现EMIF效价与寄生虫密度之间存在显著相关性，但是这些特定肽为进一步研究针对疟原虫特定表位的不同免疫反应的保护能力提供了新的可能性。
