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猪的贾第虫感染:肠道贾第虫组滋养体的检测与体外分离

Giardia infection in pigs: detection and in vitro isolation of trophozoites of the Giardia intestinalis group.

作者信息

Koudela B, Nohýnková E, Vítovec J, Pakandl M, Kulda J

机构信息

Department of Protozooses of Farm Animals, Czechoslovak Academy of Sciences, Ceské Budĕjovice.

出版信息

Parasitology. 1991 Apr;102 Pt 2:163-6. doi: 10.1017/s0031182000062442.

DOI:10.1017/s0031182000062442
PMID:1852483
Abstract

Giardia infections were detected in 14 out of 32 weaned, 1 to 2-month-old pigs, used in experiments with coccidia. Giardia trophozoites were present in the small intestine from the duodenum to the ileum with maximum numbers in the cranial part of the upper jejunum. They were localized mainly on the surface of intestinal crypts. No histological changes accompanied the infection and the infected animals were asymptomatic. Giardia trophozoites obtained from the intestines of necropsied pigs were isolated in axenic culture in a bile-supplemented TYI-S-33 medium. Morphological features of the trophozoites revealed by both light and scanning electron microscopy proved that the organism belongs to the Giardia intestinalis group.

摘要

在用于球虫实验的32头1至2月龄断奶仔猪中,有14头检测出贾第虫感染。从十二指肠到回肠的小肠中均存在贾第虫滋养体,在上段空肠的头端数量最多。它们主要定位于肠隐窝表面。感染未伴随组织学变化,受感染动物无症状。从剖检猪的肠道中获得的贾第虫滋养体在添加胆汁的TYI-S-33培养基中进行无菌培养分离。光学显微镜和扫描电子显微镜揭示的滋养体形态特征证明该生物体属于肠贾第虫组。

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